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CBR1 Antibody (YA810)

(Synonyms: 15 hydroxyprostaglandin dehydrogenase [NADP ]; 15-hydroxyprostaglandin dehydrogenase [NADP ]; Carbonyl reductase [NADPH] 1; CBR 1; CBR1; CBR1_HUMAN; CRN; NADPH dependent carbonyl reductase 1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9 ketoreduc)
Customer Review

Based on 1 Customer Validation

CBR1 Antibody (YA810) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBR1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • CBR1 Antibody (YA810) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

CBR1 Antibody (YA810) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBR1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 30 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 30 kDa
Species Reactivity Database

Entrez Gene: 873 Human

SwissProt: P16152 Human

Immunogen

Synthetic peptide corresponding to Human CBR1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102922

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CBR1 Antibody (YA810)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and SK-OV-3 (lane 6, 15 μg) using CBR1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CBR1 Antibody (YA810)
    Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CBR1 Antibody (YA810)
    Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cannabinoid receptor 1 (CBR1/CB1R) is a Gi/o-coupled G protein-coupled receptor that functions as a major regulator of endocannabinoid signaling in the central nervous system and peripheral tissues, where it modulates neurotransmitter release and synaptic activity through presynaptic mechanisms[1]. Mechanistically, CBR1 inhibits adenylyl cyclase, regulates cyclic AMP-dependent signaling, activates mitogen-activated protein kinase pathways, and modulates voltage-gated calcium and inwardly rectifying potassium channels, thereby controlling neuronal excitability and cellular responses[2]. Endocannabinoid-mediated CBR1 signaling acts as a feedback system that limits excessive synaptic transmission and contributes to neuronal plasticity and neuroprotection[3]. In disease-related experimental models, CBR1 activation promotes cell-survival pathways involving PI3K/Akt/mTORC1 signaling and brain-derived neurotrophic factor expression, supporting neuronal viability in models of striatal injury and Huntington’s disease[3]. Compared with the related cannabinoid receptor CB2, which is predominantly associated with immune tissues and immunomodulatory functions, CBR1 is highly enriched in neuronal populations and uniquely exhibits prominent ion-channel regulation[2]. For experimental applications, selective CBR1 agonists and antagonists are widely used to distinguish receptor-specific functions, investigate feeding behavior, metabolic regulation, synaptic plasticity, and neurodegenerative mechanisms, although centrally acting antagonists have shown psychiatric liabilities in clinical development[1][4].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expressed in the kidneys (protein level)

  • Isoforms & Post-Translational Modification

    P16152 has 2 isomers: P16152-1: 30375 Da (predicted); P16152-2: 18762 Da (predicted).

  • Subunit

    Monomer

  • SwissProt ID

    P16152

  • Gene ID
    873 [NCBI]
  • Synonyms

    15 hydroxyprostaglandin dehydrogenase [NADP ]; 15-hydroxyprostaglandin dehydrogenase [NADP ]; Carbonyl reductase [NADPH] 1; CBR 1; CBR1; CBR1_HUMAN; CRN; NADPH dependent carbonyl reductase 1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9 ketoreduc

  • Research Field

    Signal Transduction

[1]. Howlett AC, et al. CB1 and CB2 Receptor Pharmacology. Adv Pharmacol. 2017;80:169-206. [Content Brief]

[2]. Turu G, et al. Signal transduction of the CB1 cannabinoid receptor. J Mol Endocrinol. 2010 Feb;44(2):75-85.

[3]. Blázquez C, et al. The CB₁ cannabinoid receptor signals striatal neuroprotection via a PI3K/Akt/mTORC1/BDNF pathway. Cell Death Differ. 2015 Oct;22(10):1618-29. [Content Brief]

[4]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]

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CBR1 Antibody (YA810) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and SK-OV-3 (lane 6, 15 μg) using CBR1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CBR1 Antibody (YA810) Product Image
    1/16
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • CBR1 Antibody (YA810) Product Image
  • Experimental Validation Results for CBR1 Antibody (YA810)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and SK-OV-3 (lane 6, 15 μg) using CBR1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CBR1 Antibody (YA810)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CBR1 Antibody (YA810)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • Experimental Validation Results for CBR1 Antibody (YA810)
  • CBR1 Antibody (YA810) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), SH-SY5Y (lane 3, 15 μg), A549 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and SK-OV-3 (lane 6, 15 μg) using CBR1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CBR1 Antibody (YA810) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CBR1 Antibody (YA810) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling CBR1 with CBR1 Antibody (HY-P80590) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CBR1 Antibody (HY-P80590) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CBR1 Antibody (YA810) Product Image WB
  • CBR1 Antibody (YA810) Product Image ICC
  • CBR1 Antibody (YA810) Product Image ICC