KDM5C/Jarid1C/SMCX Antibody (YA5174)

Cat. No.: HY-P85482: Data Sheet User Guide for Antibodies
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KDM5C/Jarid1C/SMCX Antibody (YA5174) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KDM5C/Jarid1C/SMCX Antibody (YA5174) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to KDM5C/Jarid1C/SMCX.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 220 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P41229

Gene ID

8242 [NCBI]

Immunogen

Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:150

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:28262558). Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:It was expressed in all tissues tested. The highest levels were found in the brain and skeletal muscle.

Isoforms & Post-Translational Modification：P41229 has 5 isomers: P41229-1: 175720 Da (predicted); P41229-2: 175379 Da (predicted); P41229-3: 170561 Da (predicted); P41229-4: 155119 Da (predicted); P41229-5: 175592 Da (predicted).

Subunit：Part of two distinct complexes, one containing E2F6, and the other containing REST (PubMed:17468742). Interacts with ZMYND8 (PubMed:33323928)

Synonyms

DXS1272E; Histone demethylase JARID1C; JARID1C; JmjC domain containing protein SMCX; Jumonji AT rich interactive domain 1C; Jumonji, AT rich interactive domain 1C; RBP2 like; Jumonji/ARID domain-containing protein 1C; KDM5C; KDM5C_HUMAN; Lysine; K; specific demethylase 5C; Lysine-specific demethylase 5C; MRXJ; MRXSCJ; MRXSJ; Protein SmcX; Protein Xe169; rbp2 like protein; Selected cDNA on X; SMCX; Smcx homolog X chromosome; SmcX protein; SmcX protein; Smcy homolog X linked; XE169; Xe169 protein; Xe169 protein.

Documentation

User Guide for Antibodies (1077 KB)