PMS2 Antibody (YA1772)
(Synonyms: DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2)PMS2 Antibody (YA1772) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PMS2.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Liquid in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 IHC-P: 1:50-1:100 ICC/IF: 1:100-1:200 |
Product Details
PMS2 Antibody (YA1772) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PMS2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 150 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 96 kDa
Full length human recombinant protein of human PMS2 produced in 293T cell.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Liquid in PBS containing 50% glycerol, 1% BSA and 0.02% sodium azide, pH 7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PMS2 component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Possesses an ATPase activity, but in the absence of gross structural changes, ATP hydrolysis may not be necessary for proficient mismatch repair[1][2].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
P54278 has 4 isomers: P54278-1: 95797 Da (predicted); P54278-2: 51251 Da (predicted); P54278-3: 62751 Da (predicted); P54278-4: 20073 Da (predicted).
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Subunit
Heterodimer of PMS2 and MLH1 (MutL alpha); this interaction is required for the stability of both partners (PubMed:35189042). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1
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SwissProt ID
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Synonyms
DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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