XRCC1 Antibody (YA5297)

Cat. No.: HY-P85605: Ficha de datos User Guide for Antibodies
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XRCC1 Antibody (YA5297) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to XRCC1.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

XRCC1 Antibody (YA5297) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to XRCC1.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Observed band size: 82 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P18887

Gene ID

7515 [NCBI]

Immunogen

Purified recombinant human XRCC1 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Pureza	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes (PubMed:11163244, PubMed:28002403). Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity (PubMed:28002403, PubMed:34102106, PubMed:34811483). Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity (PubMed:14500814, PubMed:34102106, PubMed:34811483)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:It is expressed (at the protein level) in fibroblasts, retinal pigment epithelial cells, and lymphoblasts.

Subunit：Homodimer (PubMed:16397295). Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3) (PubMed:19155274, PubMed:19336415). Interacts with APTX and APLF (PubMed:14755728, PubMed:15044383, PubMed:15380105, PubMed:17353262, PubMed:17507382). Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1 (PubMed:19934257). Interacts with (poly-ADP-ribosylated) PARP1 (PubMed:34811483)

Synonyms

DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray repair complementing defective repair in chinese hamster cells 1; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair cross complementing protein 1; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN.

Documentación

User Guide for Antibodies (1077 KB)