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CXCL5 Antibody (YA3504)

(Synonyms: AMCFII; C-X-C motif chemokine 5; Cxcl5; CXCL5_HUMAN; ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating peptide 78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; GCP2; LIX; Neutrophil activating peptide ENA 78; Neutrophil-activating peptide ENA-78; SCYB5; Scyb6; Small inducible cytokine B5; Small inducible cytokine subfamily B member 5; Small-inducible cytokine B5.)
Customer Review

Based on 1 Customer Validation

CXCL5 Antibody (YA3504) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CXCL5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CXCL5 Antibody (YA3504)
  • CXCL5 Antibody (YA3504) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50

Product Details

Description

CXCL5 Antibody (YA3504) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CXCL5.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 12 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 12 kDa
Species Reactivity Database

Entrez Gene: 6374 Human

SwissProt: P42830 Human

Immunogen

A synthesized peptide derived from human CXCL5 aa75-114.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103257

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CXCL5 Antibody (YA3504)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and Hela+PMA (lane 2, 15 μg) using CXCL5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    CXCL5 is involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes

  • Subcellular Localization

    Secreted

  • Subunit

    Monomer (PubMed:24695525). Homodimer (PubMed:24695525)

  • SwissProt ID

    P42830

  • Gene ID
    6374 [NCBI]
  • Synonyms

    AMCFII; C-X-C motif chemokine 5; Cxcl5; CXCL5_HUMAN; ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating peptide 78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; GCP2; LIX; Neutrophil activating peptide ENA 78; Neutrophil-activating peptide ENA-78; SCYB5; Scyb6; Small inducible cytokine B5; Small inducible cytokine subfamily B member 5; Small-inducible cytokine B5.

CXCL5 Antibody (YA3504) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and Hela+PMA (lane 2, 15 μg) using CXCL5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CXCL5 Antibody (YA3504) Product Image
    1/16
  • Experimental Validation Results for CXCL5 Antibody (YA3504)
  • CXCL5 Antibody (YA3504) Product Image
  • Experimental Validation Results for CXCL5 Antibody (YA3504)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and Hela+PMA (lane 2, 15 μg) using CXCL5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CXCL5 Antibody (YA3504)
  • CXCL5 Antibody (YA3504) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), and Hela+PMA (lane 2, 15 μg) using CXCL5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CXCL5 Antibody (YA3504) Product Image WB