NOS2 Antibody (YA3969)

Cat. No.: HY-P84272: Data Sheet User Guide for Antibodies
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NOS2 Antibody (YA3969) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NOS2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NOS2 Antibody (YA3969) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NOS2.

Host

Mouse

Clonality

Monoclonal Antibody

Molecular Weight

Predicted band size: 131 kDa;

Observed band size: 131 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P35228

Gene ID

4843 [NCBI]

Immunogen

Purified recombinant fragment of human NOS2 aa 997-1058.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:It is expressed in liver, retina, bone cells, and lung airway epithelial cells. It is not expressed in platelets. It is expressed in chondrocytes (PubMed:7504305) .

Induction:By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS) , TNF or IL1B/interleukin-1 beta (PubMed:7504305, PubMed:7528267) . Down-regulated by zinc due to inhibition of NF-kappa-B transactivation activity (PubMed:25180171) . By oxidatively-modified low-densitity lipoprotein (LDL (ox) ) (PubMed:25417112)

Isoforms & Post-Translational Modification：P35228 has 2 isomers: P35228-1: 131117 Da (predicted); P35228-2: 126749 Da (predicted).
Polyubiquitinated; mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation

Subunit：Homodimer (PubMed:10074942, PubMed:10409685). Interacts with NHERF1 (PubMed:12080081). Interacts with GAPDH; induced by oxidatively-modified low-densitity lipoprotein (LDL(ox)) (PubMed:25417112). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex (PubMed:25417112). Interacts with SPSB1, SPSB2 and SPSB4 (PubMed:21199876). Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4 (PubMed:21199876). Forms a complex with ASL, ASS1 and HSP90AA1; the complex regulates cell-autonomous L-arginine synthesis and citrulline recycling while channeling extracellular L-arginine to nitric oxide synthesis pathway

Synonyms

NOS; INOS; NOS2A; HEP-NOS

Documentation

User Guide for Antibodies (1077 KB)