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beta 2 Microglobulin Antibody (YA935)

(Synonyms: B2MG; Beta 2 microglobin; Beta 2 microglobulin; Beta-2-microglobulin form pI 5.3; CDABP0092; Hdcma22p)
Customer Review

Based on 1 Customer Validation

beta 2 Microglobulin Antibody (YA935) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta 2 Microglobulin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ELISA
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • beta 2 Microglobulin Antibody (YA935) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

beta 2 Microglobulin Antibody (YA935) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta 2 Microglobulin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 14 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human beta 2 Microglobulin

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103310

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), Hela (lane 2, 15 μg), Raji (lane 3, 15 μg), Jurkat (lane 4, 15 μg), Mouse kidney (lane 5, 15 μg), and Rat kidney (lane 6, 15 μg) using beta 2 Microglobulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using beta 2 Microglobulin (HY-P81260A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    Indirect ELISA analysis of B2M was performed by coating wells of a 96-well plate with 100 µl per well of B2M antigen (HY-P7625) at a concentration of 2 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of B2M antibody, starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.05 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 15 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

Background

  • Function

    beta 2 Microglobulin component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation[1].

  • Subcellular Localization

    Secreted; Cell surface

  • Subunit

    Heterodimer of an alpha chain and a beta chain. Beta-2-microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from on long-term hemodialysis. B2M alone (not in complex with HLA-I) interacts with M.tuberculosis EsxA (ESAT-6) and an EsxA-EsxB (CFP-10) complex; the tripartite complex can be detected in the host endoplasmic reticulum (PubMed:25356553). The B2M-EsxA complex can be detected with pleural tuberculosis and is stable from pH 4.0 to 8.0 and in the presence of 2M NaCl (PubMed:25356553). Forms a heterotrimer with HLA-E and a self- or a foreign peptide (PubMed:9427624). Forms a heterotrimer with HLA-G and a self-peptide (PubMed:17056715). Forms a heterotrimer with HLA-F and a self-peptide (PubMed:10605026). Forms a heterotrimer with MR1 and a metabolite antigen

  • SwissProt ID

    P61769

  • Gene ID
    567 [NCBI]
  • Synonyms

    B2MG; Beta 2 microglobin; Beta 2 microglobulin; Beta-2-microglobulin form pI 5.3; CDABP0092; Hdcma22p

  • Research Field

    Cardiovascular

beta 2 Microglobulin Antibody (YA935) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), Hela (lane 2, 15 μg), Raji (lane 3, 15 μg), Jurkat (lane 4, 15 μg), Mouse kidney (lane 5, 15 μg), and Rat kidney (lane 6, 15 μg) using beta 2 Microglobulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using beta 2 Microglobulin (HY-P81260A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Indirect ELISA analysis of B2M was performed by coating wells of a 96-well plate with 100 µl per well of B2M antigen (HY-P7625) at a concentration of 2 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of B2M antibody, starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.05 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 15 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • beta 2 Microglobulin Antibody (YA935) Product Image
    1/16
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • beta 2 Microglobulin Antibody (YA935) Product Image
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), Hela (lane 2, 15 μg), Raji (lane 3, 15 μg), Jurkat (lane 4, 15 μg), Mouse kidney (lane 5, 15 μg), and Rat kidney (lane 6, 15 μg) using beta 2 Microglobulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using beta 2 Microglobulin (HY-P81260A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
    1/1
    MedChemExpress Validation
     Indirect ELISA analysis of B2M was performed by coating wells of a 96-well plate with 100 µl per well of B2M antigen (HY-P7625) at a concentration of 2 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of B2M antibody, starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.05 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 15 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • Experimental Validation Results for beta 2 Microglobulin Antibody (YA935)
  • beta 2 Microglobulin Antibody (YA935) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U937 (lane 1, 15 μg), Hela (lane 2, 15 μg), Raji (lane 3, 15 μg), Jurkat (lane 4, 15 μg), Mouse kidney (lane 5, 15 μg), and Rat kidney (lane 6, 15 μg) using beta 2 Microglobulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • beta 2 Microglobulin Antibody (YA935) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using beta 2 Microglobulin (HY-P81260A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • beta 2 Microglobulin Antibody (YA935) Product Image
    MedChemExpress Validation

    Indirect ELISA analysis of B2M was performed by coating wells of a 96-well plate with 100 µl per well of B2M antigen (HY-P7625) at a concentration of 2 µg/mL overnight at 4 °C. Wells of the plate were washed and blocked with 1% BSA in 1× PBST buffer. The plate was then incubated with 100 µl per well of B2M antibody, starting at an initial concentration of 10,000 ng/mL and serially diluted down to 0.05 ng/mL, for 2 hours at room temperature. The plate was washed and incubated with 100 µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using TMB substrate for 15 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid, and absorbances were read on a spectrophotometer at 450 nm.

  • beta 2 Microglobulin Antibody (YA935) Product Image WB
  • beta 2 Microglobulin Antibody (YA935) Product Image WB
  • beta 2 Microglobulin Antibody (YA935) Product Image ELISA