B2M/Beta-2-microglobulin Protein, Human (His)

B2M/Beta-2-microglobulin Protein, Human (His), a recombinant human Beta-2-microglobulin produced in E. coli, has a His tag. Beta-2-microglobulin acts as a modulator of lymphocyte surface and as a potential regulator of the immune system^[1].

Beta 2-Microglobulin is a low molecular weight protein with sequence homology to immunoglobulins. Under normal conditions beta 2-microglobulin is synthesized and shed by many cells, particularly lymphocytes, and is detectable in the circulation of normal individuals. Because of its small size it is normally filtered readily at the glomerulus and is catabolized by proximal tubular cells of the kidney. Impaired renal function and hyperproduction of beta 2-microglobulin are both associated with increased serum levels^[1].

Data is not available.

Human

E. coli

N-6*His

P61769 (I21-M119)

567  [NCBI]

HHHHHHIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

Approximately 14.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against PBS, PH 7.4 or PBS, 300 mM NaCl, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. G M Bernier. beta 2-Microglobulin: structure, function and significance. Vox Sang.1980 Jun;38(6):323-7.  [Content Brief]