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TriMethyl-Histone H3 (Lys27) Antibody (YA030)

(Synonyms: H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

TriMethyl-Histone H3 (Lys27) Antibody (YA030) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TriMethyl-Histone H3 (Lys27).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:200-1:500 1:500-1:1000

Product Details

Description

TriMethyl-Histone H3 (Lys27) Antibody (YA030) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TriMethyl-Histone H3 (Lys27).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to residues surrounding Lys27 of Human Histone H3.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Methylated

Isotype

IgG

RRID

AB_3102754

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using TriMethyl-Histone H3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    TriMethyl-Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

  • SwissProt ID

    P68431

  • Gene ID
    8350 [NCBI]
  • Synonyms

    H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a

  • Research Field

    Epigenetics and Nuclear Signaling

TriMethyl-Histone H3 (Lys27) Antibody (YA030) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using TriMethyl-Histone H3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
    1/16
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using TriMethyl-Histone H3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • Experimental Validation Results for TriMethyl-Histone H3 (Lys27) Antibody (YA030)
  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using TriMethyl-Histone H3 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with TriMethyl-Histone H3 (Lys27) Antibody (HY-P80922) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image WB
  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image ICC
  • TriMethyl-Histone H3 (Lys27) Antibody (YA030) Product Image ICC