NELF-B Antibody (YA6679)

Cat. No.: HY-P86986: Ficha de datos User Guide for Antibodies
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NELF-B Antibody (YA6679) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELF-B.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

NELF-B Antibody (YA6679) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELF-B.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 66 kDa;

Observed band size: 66 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

Q8WX92

Gene ID

25920 [NCBI]

Immunogen

Recombinant protein within Human NELF-B aa 1-250 / 580.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Pureza	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:10199401). May be able to induce chromatin unfolding (PubMed:11739404). Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes (By similarity). Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules (By similarity). Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes (By similarity)|(Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II (PubMed:23884411). In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1 (PubMed:23884411)

Subcellular Localization：Nucleus

Expression：
Tissue_Specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas

Isoforms & Post-Translational Modification：Q8WX92 has two isomers: Q8WX92-1: 65697 Da (predicted); Q8WX92-2: 70057 Da (predicted).

Subunit：The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex (PubMed:12612062)

Synonyms

A730008L03Rik antibody; AB041607 antibody; AI663983 antibody; Cobra1 antibody; Cofactor of BRCA1 antibody; DKFZp586B0519 antibody; KIAA1182 antibody; Negative elongation factor B antibody; Negative elongation factor protein B antibody; Nelf b antibody; A730008L03Rik antibody; AB041607 antibody; AI663983 antibody; Cobra1 antibody; Cofactor of BRCA1 antibody; DKFZp586B0519 antibody; KIAA1182 antibody; Negative elongation factor B antibody; Negative elongation factor protein B antibody; Nelf b antibody; NELF-B antibody; NELFB antibody; RGD1307832 antibody; RP13-122B23.3 antibody; RP23-132N23.4 antibody;

Documentación

User Guide for Antibodies (1077 KB)