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Epac1 Antibody (YA459)

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Based on 1 publication(s) in Google Scholar

Epac1 Antibody (YA459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Epac1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Epac1 Antibody (YA459) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Epac1 Antibody (YA459)

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200 1:10-1:50 1:10-1:50

Product Details

Description

Epac1 Antibody (YA459) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Epac1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 104 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Epac1.AA range:874-923.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102310

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Epac1 Antibody (YA459)
    Western blot analysis of extracts from HUVEC (lane 2(20μg) and HUVEC (lane 3(40μg) using Epac1 (HY-P80120) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Epac1 Antibody (YA459)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Epac1 Antibody (YA459)
    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin

  • Subcellular Localization

    Endomembrane system

  • Expression


    Tissue_specificity:Widely expressed with highest levels in human kidney, heart, thyroid and brain, and fetal kidney

  • Subunit

    Interacts with PDE3B and PIK3R6; form a signaling complex that regulates phosphatidylinositol 3-kinase gamma in angiogenesis

  • SwissProt ID

    O95398

  • Gene ID
    10411 [NCBI]
  • Research Field

    Signal Transduction

Epac1 Antibody (YA459) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg) and HUVEC (lane 3(40μg) using Epac1 (HY-P80120) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Epac1 Antibody (YA459) Product Image
    1/16
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Epac1 Antibody (YA459) Product Image
  • Experimental Validation Results for Epac1 Antibody (YA459)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HUVEC (lane 2(20μg) and HUVEC (lane 3(40μg) using Epac1 (HY-P80120) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Epac1 Antibody (YA459)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Epac1 Antibody (YA459)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Experimental Validation Results for Epac1 Antibody (YA459)
  • Epac1 Antibody (YA459) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HUVEC (lane 2(20μg) and HUVEC (lane 3(40μg) using Epac1 (HY-P80120) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Epac1 Antibody (YA459) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Epac1 Antibody (YA459) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Epac1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Epac1 Antibody (YA459) Product Image WB
  • Epac1 Antibody (YA459) Product Image IHC-P
  • Epac1 Antibody (YA459) Product Image IHC-P