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MTF1 Antibody (YA6660)

(Synonyms: Metal regulatory transcription factor 1 antibody; Metal response element binding transcription factor 1 antibody; Metal responsive transcription factor 1 antibody; MRE binding transcription factor antibody; MRE-binding transcription factor 1 antibody; MRE-binding transcription factor antibody; MTF 1 antibody; MTF1 antibody; MTF1_HUMAN antibody; Transcription factor MTF-1 antibody; Metal regulatory transcription factor 1 antibody; Metal response element binding transcription factor 1 antibody; Metal responsive transcription factor 1 antibody; MRE binding transcription factor antibody; MRE-binding transcription factor 1 antibody; MRE-binding transcription factor antibody; MTF 1 antibody; MTF1 antibody; MTF1_HUMAN antibody; Transcription factor MTF-1 antibody; Transcription factor MTF1 antibody; Zinc regulatory factor antibody; ZRF antibody; )
Customer Review

Based on 1 Customer Validation

MTF1 Antibody (YA6660) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • MTF1 Antibody (YA6660) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:200-1:1000 1:1000

Product Details

Description

MTF1 Antibody (YA6660) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MTF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 100 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Immunogen

Recombinant protein within human MTF1 aa 251-753

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for MTF1 Antibody (YA6660)
    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MTF1 Antibody (YA6660)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling MTF1 Antibody (HY-P86967, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    MTF1 is a Zinc-dependent transcriptional regulator of cellular adaption to conditions of exposure to heavy metals. Binds to metal responsive elements (MRE) in promoters and activates the transcription of metallothionein genes like metallothionein-2/MT2A. Also regulates the expression of metalloproteases in response to intracellular zinc and functions as a catabolic regulator of cartilages[1].

  • Subcellular Localization

    Nucleus,Cytoplasm

  • Isoforms & Post-Translational Modification

    Q14872: 753 amino acids, molecular weight 80957 Da.

  • SwissProt ID

    Q14872

  • Gene ID
    4520 [NCBI]
  • Synonyms

    Metal regulatory transcription factor 1 antibody; Metal response element binding transcription factor 1 antibody; Metal responsive transcription factor 1 antibody; MRE binding transcription factor antibody; MRE-binding transcription factor 1 antibody; MRE-binding transcription factor antibody; MTF 1 antibody; MTF1 antibody; MTF1_HUMAN antibody; Transcription factor MTF-1 antibody; Metal regulatory transcription factor 1 antibody; Metal response element binding transcription factor 1 antibody; Metal responsive transcription factor 1 antibody; MRE binding transcription factor antibody; MRE-binding transcription factor 1 antibody; MRE-binding transcription factor antibody; MTF 1 antibody; MTF1 antibody; MTF1_HUMAN antibody; Transcription factor MTF-1 antibody; Transcription factor MTF1 antibody; Zinc regulatory factor antibody; ZRF antibody;

MTF1 Antibody (YA6660) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling MTF1 Antibody (HY-P86967, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MTF1 Antibody (YA6660) Product Image
    1/16
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • MTF1 Antibody (YA6660) Product Image
  • Experimental Validation Results for MTF1 Antibody (YA6660)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MTF1 Antibody (YA6660)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling MTF1 Antibody (HY-P86967, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • Experimental Validation Results for MTF1 Antibody (YA6660)
  • MTF1 Antibody (YA6660) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from THP-1 (lane 2), HeLa (lane 3), 293T (lane 4), A549 (lane 5), and U87 (lane 6) using MTF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MTF1 Antibody (YA6660) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling MTF1 Antibody (HY-P86967, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • MTF1 Antibody (YA6660) Product Image WB
  • MTF1 Antibody (YA6660) Product Image FC