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LATS1 Antibody (YA5996)

(Synonyms: Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts; )
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Based on 1 publication(s) in Google Scholar

LATS1 Antibody (YA5996) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LATS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • LATS1 Antibody (YA5996) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

LATS1 Antibody (YA5996) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LATS1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 127 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719115

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for LATS1 Antibody (YA5996)
    Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LATS1 Antibody (YA5996)
    Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Large Tumor Suppressor 1 (LATS1) is a serine/threonine kinase and a central tumor suppressor that maintains cellular homeostasis by regulating cell proliferation, apoptosis, genomic stability, and cytoskeletal dynamics[1][2]. Within the Hippo signaling pathway, LATS1 functions together with its paralog LATS2 as a core kinase that negatively regulates the transcriptional co-activators YAP and TAZ, thereby restricting oncogenic transcriptional programs and tumor development[1]. Mechanistically, LATS1-mediated signaling integrates growth-control pathways with additional tumor-suppressive networks, including interactions with p53-associated stress responses and cell-cycle regulatory mechanisms[1]. In cancer-related contexts, reduced expression or functional impairment of LATS1 has been associated with multiple malignancies, and experimental studies demonstrate that loss of LATS pathway activity promotes tumorigenesis and contributes to breast cancer progression[1][3]. Beyond canonical Hippo signaling, LATS1 also functions as an actin-binding protein and negative regulator of actin polymerization, linking tumor suppression to cytoskeletal organization, cell migration, and cell-spreading processes[2]. Compared with the closely related isoform LATS2, accumulating evidence indicates that LATS1 and LATS2 possess both overlapping and nonredundant tumor-suppressive functions, with distinct contributions to mammary tumor phenotypes and cancer-associated biological programs[1][3]. For experimental applications, selective pharmacological inhibitors of LATS1/2 have recently been developed as chemical tools to interrogate Hippo-YAP pathway regulation and kinase-dependent signaling mechanisms in cellular models[4].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Midbody; Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body

  • Expression


    Tissue_specificity:It was expressed in all adult tissues tested, except for the lungs and kidneys.

  • Isoforms & Post-Translational Modification

    O95835 has 2 isomers: O95835-1: 126870 Da (predicted); O95835-2: 76193 Da (predicted).
    Autophosphorylated and phosphorylated during M-phase of the cell cycle (PubMed:10518011, PubMed:15122335, PubMed:9988268). Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation (PubMed:15688006). Phosphorylated by MAP4Ks; in parallel to STK3/MST2 and resulting to its activation (PubMed:26437443). Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy (PubMed:19927127)

  • Subunit

    Complexes with CDK1 in early mitosis (PubMed:9988268). LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity (PubMed:9988268). Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis (PubMed:10831611). Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis (PubMed:15220930). Interacts (via PPxY motif 2) with YAP1 (via WW domains) (PubMed:18158288). Interacts with MOB1A and MOB1B (PubMed:19739119). Interacts with LIMD1, WTIP and AJUBA (PubMed:20303269). Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:28068668). Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5 (PubMed:28087714). Interacts with SCRIB in the presence of DLG5 (PubMed:28169360). Interacts with WWTR1/TAZ (By similarity). Interacts with WWC1, WWC2 and WWC3 (via their WW domains) (PubMed:24682284)

  • SwissProt ID

    O95835

  • Gene ID
    9113 [NCBI]
  • Synonyms

    Serine/threonine-protein kinase LATS1; Large tumor suppressor homolog 1; WARTS protein kinase; h-warts;

References

[1]. Furth N, et al. The LATS1 and LATS2 tumor suppressors: beyond the Hippo pathway. Cell Death Differ. 2017 Sep;24(9):1488-1501. [Content Brief]

[2]. Visser-Grieve S, et al. LATS1 tumor suppressor is a novel actin-binding protein and negative regulator of actin polymerization. Cell Res. 2011 Oct;21(10):1513-6. [Content Brief]

[3]. Furth N, et al. LATS1 and LATS2 suppress breast cancer progression by maintaining cell identity and metabolic state. Life Sci Alliance. 2018 Oct 30;1(5):e201800171. [Content Brief]

[4]. Namoto K, et al. Discovery and Optimization of Selective Inhibitors of Large Tumor Suppressor Kinases LATS1 and 2 for In Vivo Investigation of the Hippo-YAP Pathway in Tissue Regeneration. J Med Chem. 2025 Jul 10;68(13):13591-13608. [Content Brief]

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LATS1 Antibody (YA5996) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LATS1 Antibody (YA5996) Product Image
    1/16
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • LATS1 Antibody (YA5996) Product Image
  • Experimental Validation Results for LATS1 Antibody (YA5996)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LATS1 Antibody (YA5996)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • Experimental Validation Results for LATS1 Antibody (YA5996)
  • LATS1 Antibody (YA5996) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LATS1 Antibody (YA5996) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling LATS1 with LATS1 Antibody (HY-P86304) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with LATS1 Antibody (HY-P86304) at 1/400 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • LATS1 Antibody (YA5996) Product Image ICC
  • LATS1 Antibody (YA5996) Product Image ICC