Nephrin Antibody (YA3472)

Cat. No.: HY-P83743: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Nephrin Antibody (YA3472) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nephrin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Nephrin Antibody (YA3472) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Nephrin Antibody (YA3472) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nephrin.

Host

Rabbit

Clonality

Polyclonal,Recombinant

Molecular Weight

Predicted band size: 135 kDa;

Observed band size: 200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60500

Gene ID

4868 [NCBI]

Immunogen

Recombinant protein within human Nephrin aa 1-1,050.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:3000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200-1:1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500

Purity	Affinity Purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Specifically expressed in podocytes of kidney glomeruli
Positive sample: Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Isoforms & Post-Translational Modification：O60500 has 2 isomers: O60500-1: 134742 Da (predicted); O60500-2: 130546 Da (predicted).
Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1 (By similarity). Tyrosine phosphorylation is reduced by high glucose levels (PubMed:28955049). Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1 (PubMed:28955049)

Subunit：Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL1/NEPH1 and KIRREL2; the interaction with KIRREL1 is dependent on KIRREL1 glycosylation. Interacts with KIRREL3. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1 (By similarity). Interacts with NPHS2 (PubMed:11562357). Interacts with phosphatidylinositol 3-kinase regulatory subunit PIK3R1; the interaction is reduced by high glucose levels (PubMed:28955049)

RRID

AB_3718739

Database

Entrez Gene: 4868 Human ; 54631 Mouse ; 64563 Rat

SwissProt: O60500 Human ; Q9QZS7 Mouse ; Q9R044 Rat

OMIM: 256300 Human

Synonyms

CNF antibody; Nephrin antibody; Nephrosis 1 congenital Finnish type antibody; Nephrosis 1, congenital, Finnish type (nephrin) antibody; NPHN antibody; NPHN_HUMAN antibody; NPHS 1 antibody; Nphs1 antibody; Renal glomerulus specific cell adhesion receptor antibody; Renal glomerulus-specific cell adhesion receptor antibody

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Nephrin Antibody (YA3472) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nephrin Antibody (YA3472)

Nephrin Antibody (YA3472) Featured Recommendations:

Product Detail

Background

Documentation

Nephrin Antibody (YA3472) Related Classifications

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