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Nephrin Antibody (YA3472)

(Synonyms: CNF antibody; Nephrin antibody; Nephrosis 1 congenital Finnish type antibody; Nephrosis 1, congenital, Finnish type (nephrin) antibody; NPHN antibody; NPHN_HUMAN antibody; NPHS 1 antibody; Nphs1 antibody; Renal glomerulus specific cell adhesion receptor antibody; Renal glomerulus-specific cell adhesion receptor antibody)
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Nephrin Antibody (YA3472) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nephrin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:1000 1:3000 1:200-1:1000 1:500

Product Details

Description

Nephrin Antibody (YA3472) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nephrin.

  • Host Rabbit
  • Clonality Polyclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 200 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 135 kDa
Species Reactivity Database
Immunogen

Recombinant protein within human Nephrin aa 1-1,050.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718739

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Nephrin is a Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Specifically expressed in podocytes of kidney glomeruli
    Positive sample: Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

  • Isoforms & Post-Translational Modification

    O60500 has 2 isomers: O60500-1: 134742 Da (predicted); O60500-2: 130546 Da (predicted).
    Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1 (By similarity). Tyrosine phosphorylation is reduced by high glucose levels (PubMed:28955049). Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1 (PubMed:28955049)

  • Subunit

    Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL1/NEPH1 and KIRREL2; the interaction with KIRREL1 is dependent on KIRREL1 glycosylation. Interacts with KIRREL3. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1 (By similarity). Interacts with NPHS2 (PubMed:11562357). Interacts with phosphatidylinositol 3-kinase regulatory subunit PIK3R1; the interaction is reduced by high glucose levels (PubMed:28955049)

  • SwissProt ID

    O60500

  • Gene ID
    4868 [NCBI]
  • Synonyms

    CNF antibody; Nephrin antibody; Nephrosis 1 congenital Finnish type antibody; Nephrosis 1, congenital, Finnish type (nephrin) antibody; NPHN antibody; NPHN_HUMAN antibody; NPHS 1 antibody; Nphs1 antibody; Renal glomerulus specific cell adhesion receptor antibody; Renal glomerulus-specific cell adhesion receptor antibody

Nephrin Antibody (YA3472) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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