hnRNP C1/C2 Antibody (YA5576)

Cat. No.: HY-P85884: Data Sheet User Guide for Antibodies
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hnRNP C1/C2 Antibody (YA5576) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to hnRNP C1/C2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

hnRNP C1/C2 Antibody (YA5576) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to hnRNP C1/C2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 33 kDa;

Observed band size: 38,42 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey, Rabbit, Orangutan

SwissProt ID

P07910

Gene ID

3183 [NCBI]

Immunogen

Synthesized peptide derived from human hnRNP C1/2. AA range:200-300

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of HeLa cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides (PubMed:8264621). May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671)

Subcellular Localization：Nucleus

Isoforms & Post-Translational Modification：P07910 has 4 isomers: P07910-1: 33670 Da (predicted); P07910-2: 32338 Da (predicted); P07910-3: 25230 Da (predicted); P07910-4: 27822 Da (predicted).
Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide;Sumoylated. Sumoylation reduces affinity for mRNA;Ubiquitinated and degraded after nucleo-cytoplasmic transport by YWHAE

Subunit：Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with DHX9; this interaction is direct, enhanced probably by their concomitant binding to RNA and mediates the attachment to actin filaments (PubMed:11687588). Interacts with PPIA/CYPA (PubMed:25678563). Interacts with YWHAE (PubMed:37599448)

Synonyms

HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2

Documentation

User Guide for Antibodies (1077 KB)