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hnRNP C1/C2 Antibody (YA5576)

(Synonyms: HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2)

hnRNP C1/C2 Antibody (YA5576) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to hnRNP C1/C2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey, Rabbit, Orangutan

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • hnRNP C1/C2 Antibody (YA5576) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:1000-5000

Product Details

Description

hnRNP C1/C2 Antibody (YA5576) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to hnRNP C1/C2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Rabbit, Orangutan
  • Observed Molecular Weight
    Observed band size: 38,42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Immunogen

Synthesized peptide derived from human hnRNP C1/2. AA range:200-300

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
    Immunocytochemistry analysis of HeLa cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
    Immunocytochemistry analysis of MCF-7 cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    hnRNP C1/C2 binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. N6-methyladenosine (m6A) has been shown to alter the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch', facilitating binding of HNRNPC, leading to regulation of mRNA splicing[1][2][3][4][5].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P07910 has 4 isomers: P07910-1: 33670 Da (predicted); P07910-2: 32338 Da (predicted); P07910-3: 25230 Da (predicted); P07910-4: 27822 Da (predicted).
    Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide;Sumoylated. Sumoylation reduces affinity for mRNA;Ubiquitinated and degraded after nucleo-cytoplasmic transport by YWHAE

  • Subunit

    Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 tetramers with bound pre-mRNA gives rise to a 19S complex that interacts with HNRNPA2B1 tetramers. Component of the 40S hnRNP particle. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with DHX9; this interaction is direct, enhanced probably by their concomitant binding to RNA and mediates the attachment to actin filaments (PubMed:11687588). Interacts with PPIA/CYPA (PubMed:25678563). Interacts with YWHAE (PubMed:37599448)

  • SwissProt ID

    P07910

  • Gene ID
    3183 [NCBI]
  • Synonyms

    HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2

hnRNP C1/C2 Antibody (YA5576) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • hnRNP C1/C2 Antibody (YA5576) Product Image
    1/16
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • hnRNP C1/C2 Antibody (YA5576) Product Image
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • Experimental Validation Results for hnRNP C1/C2 Antibody (YA5576)
  • hnRNP C1/C2 Antibody (YA5576) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • hnRNP C1/C2 Antibody (YA5576) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling hnRNP C1/C2 with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with hnRNP C1/C2 (PTR1336) mouse mAb (HY-P82129) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • hnRNP C1/C2 Antibody (YA5576) Product Image ICC
  • hnRNP C1/C2 Antibody (YA5576) Product Image ICC