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SRD5A2 Antibody (YA3540)

(Synonyms: 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody; 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody; 5 alpha SR2 antibody; 5 alpha-SR2 antibody; 5ART2 antibody; e II 5 alpha reductase antibody; EC antibody; MGC138457 antibody; Micropenis, included antibody; S5A2_HUMAN antibody; S5AR 2 antibody; SR type 2 antibody; SRD5A2 antibody; Steroid 5 alpha reductase 2 antibody; Steroid 5-alpha-reductase 2 antibody; Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody; Type II 5-alpha reductase antibody; )
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Based on 1 Customer Validation

SRD5A2 Antibody (YA3540) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SRD5A2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SRD5A2 Antibody (YA3540)
  • SRD5A2 Antibody (YA3540) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

SRD5A2 Antibody (YA3540) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SRD5A2.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human SRD5A2 aa1-70.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103274

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SRD5A2 Antibody (YA3540)
    Western blot analysis of extracts from HepG2 (lane 2(20μg), MCF-7 (lane 3(20μg), SH-SY5Y (lane 4(20μg), using SRD5A2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology

  • Subcellular Localization

    Microsome membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in high levels in the prostate and many other androgen-sensitive tissues

  • SwissProt ID

    P31213

  • Gene ID
    6716 [NCBI]
  • Synonyms

    3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody; 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody; 5 alpha SR2 antibody; 5 alpha-SR2 antibody; 5ART2 antibody; e II 5 alpha reductase antibody; EC antibody; MGC138457 antibody; Micropenis, included antibody; S5A2_HUMAN antibody; S5AR 2 antibody; SR type 2 antibody; SRD5A2 antibody; Steroid 5 alpha reductase 2 antibody; Steroid 5-alpha-reductase 2 antibody; Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody; Type II 5-alpha reductase antibody;

SRD5A2 Antibody (YA3540) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), MCF-7 (lane 3(20μg), SH-SY5Y (lane 4(20μg), using SRD5A2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SRD5A2 Antibody (YA3540) Product Image
    1/16
  • Experimental Validation Results for SRD5A2 Antibody (YA3540)
  • SRD5A2 Antibody (YA3540) Product Image
  • Experimental Validation Results for SRD5A2 Antibody (YA3540)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg), MCF-7 (lane 3(20μg), SH-SY5Y (lane 4(20μg), using SRD5A2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SRD5A2 Antibody (YA3540)
  • SRD5A2 Antibody (YA3540) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg), MCF-7 (lane 3(20μg), SH-SY5Y (lane 4(20μg), using SRD5A2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SRD5A2 Antibody (YA3540) Product Image WB