IRF2 Antibody (YA3130)(PBS only)

Cat. No.: HY-P83385A: Data Sheet User Guide for Antibodies
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IRF2 Antibody (YA3130) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IRF2 Antibody (YA3130) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRF2.

Host

Rabbit

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P14316

Gene ID

3660 [NCBI]

Immunogen

A synthesized peptide derived from human IRF2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:20-1:50
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500

Purity	Affinity Chromatography	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed throughout the epithelium of the colon. Also expressed in lamina propria

Induction:By viruses and IFN

Isoforms & Post-Translational Modification：P14316 has 2 isomers: P14316-1: 39354 Da (predicted); P14316-2: 39156 Da (predicted).
Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity;The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter

Subunit：Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity

Research Field

Immunology

Synonyms

Interferon regulatory factor 2; IRF 2

Documentation

User Guide for Antibodies (1077 KB)