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Arginase-1 Antibody (YA6101)

(Synonyms: Arginase-1, Liver-type arginase, Type I arginase, ARG1)
Customer Review

Based on 1 Customer Validation

Arginase-1 Antibody (YA6101) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Arginase-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Arginase-1 Antibody (YA6101) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Arginase-1 Antibody (YA6101) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Arginase-1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody (HY-P86409) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86409, 1/2000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Western blot analysis of extracts from HepG2(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using Arginase-1 Antibody (HY-P86409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded human Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded Mouse Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded rat Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Arginase-1 is key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure[1][2][3].

  • Subcellular Localization

    Cytoplasm; Cytoplasmic granule

  • Expression


    Tissue_specificity:In the immune system, it was initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMN]) (PubMed:15546957) . It has also been detected in macrophages of mycobacterial granulomas (PubMed:23749634) . During lung disease, it is expressed in type 2 innate lymphocytes (ILC2) (PubMed:27043409) .

    Induction:By arginine or homoarginine

  • Isoforms & Post-Translational Modification

    P05089 has 3 isomers: P05089-1: 34735 Da (predicted); P05089-2: 35664 Da (predicted); P05089-3: 25356 Da (predicted).

  • Subunit

    Homotrimer (PubMed:16141327, PubMed:17469833, PubMed:17562323, PubMed:18802628, PubMed:2241902). Interacts with CMTM6 (PubMed:28813417)

  • SwissProt ID

    P05089

  • Gene ID
    383 [NCBI]
  • Synonyms

    Arginase-1, Liver-type arginase, Type I arginase, ARG1

Arginase-1 Antibody (YA6101) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody (HY-P86409) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86409, 1/2000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using Arginase-1 Antibody (HY-P86409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    1/16
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Arginase-1 Antibody (YA6101) Product Image
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody (HY-P86409) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86409, 1/2000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using Arginase-1 Antibody (HY-P86409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat testis tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Experimental Validation Results for Arginase-1 Antibody (YA6101)
  • Arginase-1 Antibody (YA6101) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody (HY-P86409) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Arginase-1 Antibody (YA6101) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Raw 264.7 (lane 2(20μg)) 、MOLT-4 (lane 3(20μg)) 、NIH-3T3 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、Human liver (lane 6(20μg)) 、Mouse liver (lane 7(20μg)) and Rat liver (lane 8(20μg)) using Arginase-1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86409, 1/2000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) , RAW264.7(lane 5(20ug) using Arginase-1 Antibody (HY-P86409) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Arginase-1 Antibody (YA6101) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Arginase-1 Antibody (YA6101) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Arginase-1 Antibody (YA6101) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat Liver tissue using Arginase-1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86409, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Arginase-1 Antibody (HY-P86409, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Arginase-1 Antibody (YA6101) Product Image WB
  • Arginase-1 Antibody (YA6101) Product Image WB
  • Arginase-1 Antibody (YA6101) Product Image WB
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P
  • Arginase-1 Antibody (YA6101) Product Image IHC-P