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Beta Actin (Fruit Fly) Antibody

(Synonyms: ACT1_DROME; Dro-actin; Actin 5C; Act5C; Fruit Fly Actin; β-actin; β actin; )
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Based on 1 Customer Validation

Beta Actin (Fruit Fly) Antibody is a non-conjugated and Rabbit origined IgG polyclonal antibody, targeting to Beta Actin (Fruit Fly). It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Fruit Fly

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Beta Actin (Fruit Fly) Antibody
  • Beta Actin (Fruit Fly) Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-5000

Product Details

Description

Beta Actin (Fruit Fly) Antibody is a non-conjugated and Rabbit origined IgG polyclonal antibody, targeting to Beta Actin (Fruit Fly). It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Fruit Fly
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 31521 Fruit

SwissProt: P10987 Fruit

Immunogen

Recombinant Fruit Fly Actin.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103122

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beta Actin (Fruit Fly) Antibody
    Western blot analysis of extracts from Fruit Fly (lane 2, 20ug) using Beta Actin (Fruit Fly) Antibody (HY-P81254). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Beta Actin is an Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells; Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Subunit

    Interacts with Rab6

  • SwissProt ID

    P10987

  • Gene ID
    31521 [NCBI]
  • Synonyms

    ACT1_DROME; Dro-actin; Actin 5C; Act5C; Fruit Fly Actin; β-actin; β actin;

Beta Actin (Fruit Fly) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Fruit Fly (lane 2, 20ug) using Beta Actin (Fruit Fly) Antibody (HY-P81254). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Beta Actin (Fruit Fly) Antibody Product Image
    1/16
  • Experimental Validation Results for Beta Actin (Fruit Fly) Antibody
  • Beta Actin (Fruit Fly) Antibody Product Image
  • Experimental Validation Results for Beta Actin (Fruit Fly) Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Fruit Fly (lane 2, 20ug) using Beta Actin (Fruit Fly) Antibody (HY-P81254). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beta Actin (Fruit Fly) Antibody
  • Beta Actin (Fruit Fly) Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Fruit Fly (lane 2, 20ug) using Beta Actin (Fruit Fly) Antibody (HY-P81254). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Beta Actin (Fruit Fly) Antibody Product Image WB