PKA C beta Antibody
(Synonyms: cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB)PKA C beta Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PKA C beta.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
PKA C beta Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PKA C beta.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 43 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 40 kDa
Synthetic peptide corresponding to the C-term region of human PKA C beta.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PKA C beta mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR[1][2][3][4].
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Subcellular Localization
Cytoplasm; Cell membrane; Membrane; Nucleus
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Expression
Tissue_Specificity: Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. -
Isoforms & Post-Translational Modification
PKA C beta has 10 isoforms, P22694-1: amino acid length is 351, molecular weight is 40623 Da (predicted); P22694-2: amino acid length is 398, molecular weight is 46236 Da (predicted); P22694-3: amino acid length is 339, molecular weight is 39477 Da (predicted); P22694-4: amino acid length is 338, molecular weight is 39379 Da (predicted); P22694-5: amino acid length is 354, molecular weight is 40947 Da (predicted); P22694-6: amino acid length is 357, molecular weight is 41296 Da (predicted); P22694-7: amino acid length is 355, molecular weight is 41046 Da (predicted); P22694-8: amino acid length is 257, molecular weight is 29696 Da (predicted); P22694-9: amino acid length is 358, molecular weight is 41395 Da (predicted); P22694-10: amino acid length is 321, molecular weight is 36979 Da (predicted).
Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. -
Subunit
A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits.
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SwissProt ID
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Synonyms
cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB
Documentation
[1]. Wu KJ, et al. c-MYC activates protein kinase A (PKA) by direct transcriptional activation of the PKA catalytic subunit beta (PKA-Cbeta) gene. Oncogene. 2002 Nov 7;21(51):7872-82. [Content Brief]
[2]. Lignitto L, et al. Control of PKA stability and signalling by the RING ligase praja2. Nat Cell Biol. 2011 Apr;13(4):412-22. [Content Brief]
[3]. Jewell JL, et al. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor. Elife. 2019 May 21;8:. [Content Brief]
[4]. Aksaas AK, et al. G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A. J Mol Signal. 2011 Aug 31;6:10. [Content Brief]
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