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TEF1/TEAD-1 Antibody (YA1954)

(Synonyms: TEAD1; TCF13; TEF1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13)
Customer Review

Based on 1 Customer Validation

TEF1/TEAD-1 Antibody (YA1954) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TEF1/TEAD-1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    1.Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
    2.Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • TEF1/TEAD-1 Antibody (YA1954) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50

Product Details

Description

TEF1/TEAD-1 Antibody (YA1954) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TEF1/TEAD-1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TEAD1 aa201-248/426.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104322

Product Properties

  • Appearance

    Solution

  • Formulation

    1.Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
    2.Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
    Please refer to the lot-specific COA for specific buffer information.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Western blot analysis of extracts from A549 (lane2(20μg), Hela (lane3(20μg), C2C12 (lane4(20μg) and SiHa (lane5(20μg) using TEF1 Antibody (HY-P82209). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Immunohistochemical analysis of paraffin-embedded human melanoma tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

Background

  • Function

    TEF1/TEAD-1 transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is primarily expressed in skeletal muscle. Expression levels are lower in the pancreas, placenta, and heart.

  • Isoforms & Post-Translational Modification

    P28347 has 2 isomers: P28347-1: 47946 Da (predicted); P28347-2: 40000 Da (predicted).
    Lactylation by AARS1 promotes nuclear localization and stabilization of YAP1, leading to increased Hippo signaling pathway (PubMed:38512451). Delactylated by SIRT1 (PubMed:38512451)

  • Subunit

    Interacts with YAP1 and WWTR1/TAZ

  • SwissProt ID

    P28347

  • Gene ID
    7003 [NCBI]
  • Synonyms

    TEAD1; TCF13; TEF1; Transcriptional enhancer factor TEF-1; NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13

  • Research Field

    Epigenetics and Nuclear Signaling

TEF1/TEAD-1 Antibody (YA1954) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2(20μg), Hela (lane3(20μg), C2C12 (lane4(20μg) and SiHa (lane5(20μg) using TEF1 Antibody (HY-P82209). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human melanoma tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    1/16
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • TEF1/TEAD-1 Antibody (YA1954) Product Image
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2(20μg), Hela (lane3(20μg), C2C12 (lane4(20μg) and SiHa (lane5(20μg) using TEF1 Antibody (HY-P82209). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human melanoma tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • Experimental Validation Results for TEF1/TEAD-1 Antibody (YA1954)
  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane2(20μg), Hela (lane3(20μg), C2C12 (lane4(20μg) and SiHa (lane5(20μg) using TEF1 Antibody (HY-P82209). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue using TEF1/TEAD-1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82209, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human tonsil tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human placenta tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human lung tissue using TEF1/TEAD-1 Antibody (YA1954). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82209, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

  • TEF1/TEAD-1 Antibody (YA1954) Product Image WB
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image IHC-P
  • TEF1/TEAD-1 Antibody (YA1954) Product Image mIHC
  • TEF1/TEAD-1 Antibody (YA1954) Product Image mIHC
  • TEF1/TEAD-1 Antibody (YA1954) Product Image mIHC
  • TEF1/TEAD-1 Antibody (YA1954) Product Image mIHC