Wnt2b Antibody (YA2424)(PBS only)

Cat. No.: HY-P82679A: Data Sheet User Guide for Antibodies
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Wnt2b Antibody (YA2424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2b.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Wnt2b Antibody (YA2424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2b.

Host

Rabbit

Molecular Weight

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q93097

Gene ID

7482 [NCBI]

Immunogen

A synthetic peptide of human Wnt2b aa287-300.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development

Subcellular Localization：Secreted, extracellular space, extracellular matrix; Secreted

Expression：
Tissue_specificity:Isoform 1 is expressed in human heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the human brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines

Isoforms & Post-Translational Modification：Q93097 has 2 isomers: Q93097-1: 43770 Da (predicted); Q93097-2: 41830 Da (predicted).
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition

Subunit：Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720).

Research Field

Stem Cells

Synonyms

WNT13; Wnt2b; XWNT2

Documentation

User Guide for Antibodies (1077 KB)