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Wnt2b Antibody (YA2424)(PBS only)

(Synonyms: WNT13; Wnt2b; XWNT2)

Wnt2b Antibody (YA2424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2b.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Wnt2b Antibody (YA2424)(PBS only)
  • Wnt2b Antibody (YA2424)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Wnt2b Antibody (YA2424) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt2b.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Immunogen

A synthetic peptide of human Wnt2b aa287-300.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wnt2b Antibody (YA2424)(PBS only)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562(lane 2, 20 μg), and C6 (lane 3, 20 μg) using Wnt2b Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Wnt2b is a Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix; Secreted

  • Expression


    Tissue_specificity:Isoform 1 is expressed in human heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the human brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines

  • Isoforms & Post-Translational Modification

    Q93097 has 2 isomers: Q93097-1: 43770 Da (predicted); Q93097-2: 41830 Da (predicted).
    Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition

  • Subunit

    Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720).

  • SwissProt ID

    Q93097

  • Gene ID
    7482 [NCBI]
  • Synonyms

    WNT13; Wnt2b; XWNT2

  • Research Field

    Stem Cells

Wnt2b Antibody (YA2424)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562(lane 2, 20 μg), and C6 (lane 3, 20 μg) using Wnt2b Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Wnt2b Antibody (YA2424)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Wnt2b Antibody (YA2424)(PBS only)
  • Wnt2b Antibody (YA2424)(PBS only) Product Image
  • Experimental Validation Results for Wnt2b Antibody (YA2424)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562(lane 2, 20 μg), and C6 (lane 3, 20 μg) using Wnt2b Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Wnt2b Antibody (YA2424)(PBS only)
  • Wnt2b Antibody (YA2424)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562(lane 2, 20 μg), and C6 (lane 3, 20 μg) using Wnt2b Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Wnt2b Antibody (YA2424)(PBS only) Product Image WB