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MUC2 Antibody (YA3529)

(Synonyms: SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2)
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Based on 1 Customer Validation

MUC2 Antibody (YA3529) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MUC2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for MUC2 Antibody (YA3529)
  • MUC2 Antibody (YA3529) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50 1:50-1:100

Product Details

Description

MUC2 Antibody (YA3529) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to MUC2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 540 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human MUC2 aa5000-5100.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Verification Images

  • Experimental Validation Results for MUC2 Antibody (YA3529)
    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2) and HeLa (lane 3) using MUC2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    MUC2 encodes the major gel-forming mucin produced by intestinal goblet cells and constitutes the structural backbone of the gastrointestinal mucus barrier, where it physically separates luminal microorganisms from the epithelial surface and supports mucosal homeostasis[4][5]. Mechanistically, MUC2 polymers form highly organized mucus networks that generate the characteristic inner and outer colonic mucus layers, thereby regulating microbial access, antigen exposure, and host defense responses[1][2]. Through these barrier functions, MUC2 contributes to intestinal immune regulation and maintenance of a stable microbiota, while defective synthesis, secretion, or organization of MUC2 compromises barrier integrity and promotes epithelial contact with bacteria[5][6]. In disease settings, dysregulated MUC2 production has been closely associated with inflammatory bowel disease and chronic intestinal inflammation, and experimental studies demonstrate that impairment of the MUC2 barrier increases susceptibility to colitis and inflammation-related pathology[2][6][3]. Compared with related membrane-associated mucins such as MUC1, MUC3, and MUC17, MUC2 is distinguished by its role as a secreted polymeric mucin that forms the bulk extracellular mucus scaffold rather than functioning primarily at the epithelial cell surface[3]. For experimental applications, MUC2 serves as a widely used biomarker of goblet cell differentiation, mucus barrier integrity, and host-microbiota interactions, making it a valuable target for studies investigating intestinal barrier dysfunction, mucosal immunity, and therapeutic strategies aimed at restoring mucus homeostasis[4][6].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Colon, small intestine, colon tumor, bronchi, cervix, and gallbladder

  • Subunit

    Homomultimer; disulfide-linked (PubMed:12374796, PubMed:31310764, PubMed:33031746, PubMed:35377815). The N- and C-terminus mediate their assembly into higher order structures to form filaments (PubMed:33031746, PubMed:35377815). The CTCK domains of two polypeptides associate in the endoplasmic reticulum to generate intermolecularly disulfide-bonded dimers (By similarity). These dimers progress to the Golgi apparatus, which is a more acidic environment than the endoplasmic reticulum (PubMed:33031746). Under acidic conditions, the N-termini form non-covalent intermolecular interactions that juxtapose assemblies of the third VWD domain (VWD3) from different CTCK-linked dimers (PubMed:33031746). The VWD3 assemblies then become disulfide bonded to one another to produce long, disulfide-linked polymers that remain highly compact until secretion (PubMed:33031746). Interacts with FCGBP (PubMed:19432394). Interacts with AGR2; disulfide-linked (PubMed:19359471)

  • SwissProt ID

    Q02817

  • Gene ID
    4583 [NCBI]
  • Synonyms

    SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

  • Research Field

    Tags & Cell Markers

[1]. Birchenough GMH, et al. New developments in goblet cell mucus secretion and function. Mucosal Immunol. 2015;8(4):712-719.

[2]. Breugelmans T, et al. The role of mucins in gastrointestinal barrier function during health and disease. Lancet Gastroenterol Hepatol. 2022;7(5):455-471.

[3]. Liu Y, et al. Orchestration of MUC2 - The key regulatory target of gut barrier and homeostasis: A review. Int J Biol Macromol. 2023 May 1;236:123862. [Content Brief]

[4]. Kim YS, et al. Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroenterol Rep. 2010;12(5):319-330. [Content Brief]

[5]. Johansson ME, et al. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host-microbial interactions. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1(Suppl 1):4659-65. [Content Brief]

[6]. Yao D, et al. MUC2 and related bacterial factors: therapeutic targets for ulcerative colitis. EBioMedicine. 2021;74:103751. [Content Brief]

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MUC2 Antibody (YA3529) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2) and HeLa (lane 3) using MUC2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MUC2 Antibody (YA3529) Product Image
    1/16
  • Experimental Validation Results for MUC2 Antibody (YA3529)
  • MUC2 Antibody (YA3529) Product Image
  • Experimental Validation Results for MUC2 Antibody (YA3529)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2) and HeLa (lane 3) using MUC2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for MUC2 Antibody (YA3529)
  • MUC2 Antibody (YA3529) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2) and HeLa (lane 3) using MUC2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • MUC2 Antibody (YA3529) Product Image WB