Meckelin Antibody (YA3057)(PBS only)

Cat. No.: HY-P83312A: Data Sheet User Guide for Antibodies
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Meckelin Antibody (YA3057) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Meckelin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Meckelin Antibody (YA3057) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Meckelin.

Host

Rabbit

Molecular Weight

Predicted band size: 112 kDa;

Observed band size: 112 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q5HYA8

Gene ID

91147 [NCBI]

Immunogen

A synthetic peptide of human Meckelin aa34-47.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Required for ciliary structure and function. Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). Involved in centrosome migration to the apical cell surface during early ciliogenesis. Involved in the regulation of cilia length and appropriate number through the control of centrosome duplication. Is a key regulator of stereociliary bundle orientation (By similarity). Required for epithelial cell branching morphology. Essential for endoplasmic reticulum-associated degradation (ERAD) of surfactant protein C (SFTPC). Involved in the negative regulation of canonical Wnt signaling, and activation of the non-canonical cascade stimulated by WNT5A (PubMed:26035863). In non-canonical Wnt signaling, it may act as ROR2 coreceptor (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell projection, cilium; Cytoplasm, cytoskeleton, cilium basal body

Expression：
Tissue_specificity:Widely expressed in human tissues. Expressed at higher level in spinal cord

Subunit：Homodimer (PubMed:34731008). Part of the tectonic-like complex (also named B9 complex) (By similarity). Interacts with DNAJB9, DNAJC10 and mutated SFTPC. Interacts with SYNE2 during the early establishment of cell polarity. Interacts (via C-terminus) with FLNA. Interacts with TMEM218 (PubMed:35137054). Interacts with WNT5A (PubMed:26035863, PubMed:34731008). Interacts with ROR2 (PubMed:26035863)

Research Field

Tags & Cell Markers

Synonyms

MKS3; JBTS6; NPHP11; TNEM67; MECKELIN

Documentation

User Guide for Antibodies (1077 KB)