Ubiquitin Conjugation Factor E4 B Antibody (YA3308)(PBS only)

Cat. No.: HY-P83563A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Ubiquitin Conjugation Factor E4 B Antibody (YA3308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Conjugation Factor E4 B.

Host

Rabbit

Molecular Weight

Predicted band size: 146 kDa;

Observed band size: 146 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O95155

Gene ID

10277 [NCBI]

Immunogen

A synthetic peptide of human UBE4B aa28-40.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Expressed in differentiated myotubes (at protein level) (PubMed:17369820) . Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788) . Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788) . Almost undetectable in lung and spleen (PubMed:11802788)

Isoforms & Post-Translational Modification：O95155 has 4 isomers: O95155-1: 146185 Da (predicted); O95155-2: 133275 Da (predicted); O95155-3: 121820 Da (predicted); O95155-4: 151561 Da (predicted).
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7

Subunit：Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B

Research Field

Synonyms

E4; UFD2; HDNB1; UBOX3; UFD2A

Documentation

User Guide for Antibodies (1077 KB)