Cytokeratin 8 Antibody (YA6196)

Cat. No.: HY-P86504: Data Sheet User Guide for Antibodies
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Cytokeratin 8 Antibody (YA6196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Cytokeratin 8 Antibody (YA6196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 8.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P05787

Gene ID

3856 [NCBI]

Immunogen

Synthetic peptide within Human Cytokeratin 8 aa 321-370/483.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1,000-1:2,000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:400-1:800
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:400-1:800
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1,000-1:1,500
FC FC: Flow Cytometry	1:500-1:1,000
IP IP: Immunoprecipitation	2 µg/10 µl beads.

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle

Subcellular Localization：Cytoplasm; Nucleus, nucleoplasm; Nucleus matrix

Expression：
Tissue_specificity:Aggregates of dystrophin and actin are observed in muscle fibers at the sarcoplasmic sarcomere and sarcomere. They are also expressed in the gingival mucosa and hard palate of the oral cavity.

Isoforms & Post-Translational Modification：P05787 has 2 isomers: P05787-1: 53704 Da (predicted); P05787-2: 56608 Da (predicted).
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization;O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation;O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner

Subunit：Heterotetramer of two type I and two type II keratins (PubMed:16000376). Forms a heterodimer with KRT18 (PubMed:10852826, PubMed:24940650). Associates with KRT20 (PubMed:10973561, PubMed:16608857). Interacts with PLEC isoform 1C, when in a heterodimer with KRT18 (PubMed:24940650). Interacts with PNN (PubMed:10809736). When associated with KRT19, interacts with DMD. Interacts with TCHP (PubMed:15731013). Interacts with APEX1 (PubMed:19188445). Interacts with GPER1 (PubMed:21149639). Interacts with EPPK1 (By similarity). Interacts with PKP1 and PKP2 (PubMed:10852826)

Synonyms

CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8

Documentation

User Guide for Antibodies (1077 KB)