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  5. Acetyl-Histone H3 (Lys27) Antibody (YA642)

Acetyl-Histone H3 (Lys27) Antibody (YA642)

Cat. No.: HY-P80530
COA
SDS
User Guide for Antibodies Technical Support

Acetyl-Histone H3 (Lys27) Antibody (YA642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys27).

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Acetyl-Histone H3 (Lys27) Antibody (YA642)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

Acetyl-Histone H3 (Lys27) Antibody (YA642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys27).
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 15 kDa;
Observed band size: 15 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to residues surrounding Lys27 of Human Human Histone H3.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:500-1:5000
FC
FC: Flow Cytometry
1:200-1:1000
Sensitivity Endogenous Purity affinity purified
Conjugation Non-conjugated Modification Acetylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and M-lymph (lane 3) using Acetyl-Histone H3 (Lys27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
Subcellular Localization：Nucleus; Chromosome
Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)
RRID
Database

Entrez Gene: 8350 Human ; 8351 Human ; 8352 Human ; 8353 Human ; 8354 Human ; 8355 Human ; 8356 Human ; 8357 Human ; 8358 Human ; 8968 Human

SwissProt: P68431 Human

OMIM: 137800 Human

Research Field

Epigenetics and Nuclear Signaling
Synonyms
H3K27ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Acetyl-Histone H3 (Lys27) Antibody (YA642) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Acetyl-Histone H3 (Lys27) Antibody (YA642)
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