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Acetyl-Histone H3 (Lys14) Antibody (YA643)

(Synonyms: H3K14ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J)
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Based on 1 Customer Validation

Acetyl-Histone H3 (Lys14) Antibody (YA643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys14).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Acetyl-Histone H3 (Lys14) Antibody (YA643) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl-Histone H3 (Lys14).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to residues surrounding Lys14 of Human Human Histone H3.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG

RRID

AB_3102118

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    Western blot analysis of extracts from Raji (lane 1) and Hela (lane 2) and NIH3T3 (lane 3) using Acetyl-Histone H3 (Lys14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Acetyl-Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

  • SwissProt ID

    P68431

  • Gene ID
    8350 [NCBI]
  • Synonyms

    H3K14ac; H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J

  • Research Field

    Epigenetics and Nuclear Signaling

Acetyl-Histone H3 (Lys14) Antibody (YA643) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Raji (lane 1) and Hela (lane 2) and NIH3T3 (lane 3) using Acetyl-Histone H3 (Lys14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
    1/16
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Raji (lane 1) and Hela (lane 2) and NIH3T3 (lane 3) using Acetyl-Histone H3 (Lys14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Experimental Validation Results for Acetyl-Histone H3 (Lys14) Antibody (YA643)
  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Raji (lane 1) and Hela (lane 2) and NIH3T3 (lane 3) using Acetyl-Histone H3 (Lys14) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Acetyl-Histone H3 (Lys14) with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Acetyl-Histone H3 (Lys14) Antibody (HY-P80529) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image WB
  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image ICC
  • Acetyl-Histone H3 (Lys14) Antibody (YA643) Product Image ICC