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P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)

(Synonyms: RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70)
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Based on 1 Customer Validation

P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P70 S6 Kinase beta/SRK (phospho S371).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)
  • P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P70 S6 Kinase beta/SRK (phospho S371).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Species Reactivity Database
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser371 of human P70 S6 Kinase beta

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3104028

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)
    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and HEK293T (lane 5, 20ug) using P70 S6 Kinase beta/SRK (phospho S371) (YA1890) Antibody (HY-P82145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    P70 S6 Kinase beta/SRK phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7[1].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    Q9UBS0 has 2 isomers: Q9UBS0-1: 53455 Da (predicted); Q9UBS0-2: 16868 Da (predicted).
    Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention

  • SwissProt ID

    Q9UBS0

  • Gene ID
    6199 [NCBI]
  • Synonyms

    RPS6KB2; STK14B; Ribosomal protein S6 kinase beta-2; S6K-beta-2; S6K2; 70 kDa ribosomal protein S6 kinase 2; P70S6K2; p70-S6K 2; S6 kinase-related kinase; SRK; Serine/threonine-protein kinase 14B; p70 ribosomal S6 kinase beta; S6K-beta; p70

  • Research Field

    Signal Transduction

P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and HEK293T (lane 5, 20ug) using P70 S6 Kinase beta/SRK (phospho S371) (YA1890) Antibody (HY-P82145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Product Image
    1/16
  • Experimental Validation Results for P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)
  • P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Product Image
  • Experimental Validation Results for P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and HEK293T (lane 5, 20ug) using P70 S6 Kinase beta/SRK (phospho S371) (YA1890) Antibody (HY-P82145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890)
  • P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), C6 (lane 3, 20ug), NIH/3T3 (lane 4, 20ug) and HEK293T (lane 5, 20ug) using P70 S6 Kinase beta/SRK (phospho S371) (YA1890) Antibody (HY-P82145). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • P70 S6 Kinase beta/SRK (phospho S371) Antibody (YA1890) Product Image WB