Cyclin A2 Antibody (YA3336)

Cat. No.: HY-P83591: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Cyclin A2 Antibody (YA3336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin A2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cyclin A2 Antibody (YA3336) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Cyclin A2 Antibody (YA3336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin A2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 52,49 kDa;

Observed band size: 52,49 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P20248

Gene ID

890 [NCBI]

Immunogen

A synthesized peptide derived from human Cyclin A1/A2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A549(lane 2(20ug) , HL-60(lane 3(20ug) and Hela(lane 4(20ug) using Cyclin A1/A2 Antibody (HY-P83591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle

Subcellular Localization：Nucleus; Cytoplasm

Subunit：Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes (PubMed:1312467, PubMed:7630397, PubMed:8684460, PubMed:8756328). Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2) (PubMed:1312467). Interacts with CDK2; the interaction increases from G1 to G2 (PubMed:1312467). Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm (PubMed:17698606). Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements (PubMed:8684460). Interacts with INCA1 (PubMed:21540187)

RRID

AB_3105699

Database

Entrez Gene: 890 Human

SwissProt: P20248 Human

OMIM: 123835 Human

Research Field

Cell Biology

Synonyms

CCN1; CCNA; CCNA1; CCNA2; CT146; Cyclin-A; Cyclin-A1; Cyclin-A2

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cyclin A2 Antibody (YA3336) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cyclin A2 Antibody (YA3336)

Cyclin A2 Antibody (YA3336) Featured Recommendations:

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Background

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Cyclin A2 Antibody (YA3336) Related Classifications

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