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Cyclin A2 Antibody (YA3336)

(Synonyms: CCN1; CCNA; CCNA1; CCNA2; CT146; Cyclin-A; Cyclin-A1; Cyclin-A2)
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Based on 1 Customer Validation

Cyclin A2 Antibody (YA3336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin A2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cyclin A2 Antibody (YA3336)
  • Cyclin A2 Antibody (YA3336) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Cyclin A2 Antibody (YA3336) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin A2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52,49 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52,49 kDa
Species Reactivity Database

Entrez Gene: 890 Human

SwissProt: P20248 Human

Immunogen

A synthesized peptide derived from human Cyclin A1/A2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105699

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin A2 Antibody (YA3336)
    Western blot analysis of extracts from A549(lane 2(20ug) , HL-60(lane 3(20ug) and Hela(lane 4(20ug) using Cyclin A1/A2 Antibody (HY-P83591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Cyclin A2 is a Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Subunit

    Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes (PubMed:1312467, PubMed:7630397, PubMed:8684460, PubMed:8756328). Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2) (PubMed:1312467). Interacts with CDK2; the interaction increases from G1 to G2 (PubMed:1312467). Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm (PubMed:17698606). Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements (PubMed:8684460). Interacts with INCA1 (PubMed:21540187)

  • SwissProt ID

    P20248

  • Gene ID
    890 [NCBI]
  • Synonyms

    CCN1; CCNA; CCNA1; CCNA2; CT146; Cyclin-A; Cyclin-A1; Cyclin-A2

  • Research Field

    Cell Biology

Cyclin A2 Antibody (YA3336) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , HL-60(lane 3(20ug) and Hela(lane 4(20ug) using Cyclin A1/A2 Antibody (HY-P83591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cyclin A2 Antibody (YA3336) Product Image
    1/16
  • Experimental Validation Results for Cyclin A2 Antibody (YA3336)
  • Cyclin A2 Antibody (YA3336) Product Image
  • Experimental Validation Results for Cyclin A2 Antibody (YA3336)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20ug) , HL-60(lane 3(20ug) and Hela(lane 4(20ug) using Cyclin A1/A2 Antibody (HY-P83591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cyclin A2 Antibody (YA3336)
  • Cyclin A2 Antibody (YA3336) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20ug) , HL-60(lane 3(20ug) and Hela(lane 4(20ug) using Cyclin A1/A2 Antibody (HY-P83591) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cyclin A2 Antibody (YA3336) Product Image WB