Copy Link Print Information

Human IgG3 Antibody (YA10021)

(Synonyms: Growth hormone receptor; GH receptor; Somatotropin receptor)

Human IgG3 Antibody (YA10021) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Human IgG3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Human IgG3 Antibody (YA10021)
  • Human IgG3 Antibody (YA10021) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-2000 1:10-50

Product Details

Description

Human IgG3 Antibody (YA10021) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Human IgG3.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41 kDa
Immunogen

Synthetic peptide corresponding to human Human IgG3 protein.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Human IgG3 Antibody (YA10021)
    Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of IgG3 Fc Protein, Human (HEK293) using Human IgG3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Human IgG3 is a Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens[1][2].

  • Subcellular Localization

    Secreted; Cell membrane; Single-pass membrane protein

  • Isoforms & Post-Translational Modification

    P01860 has 2 isomers: P01860-1: 41,287 Da (predicted); P01860-2: 49,093 Da (predicted).
    N-glycosylated. The core glycan is composed of two sequential N-acetylglucosamine (GlcNAc) moieties followed by a central mannose (Man) from which two additional Man residues branch out (alpha1,3 and alpha1,6 antennae) each capped with a GlcNAc. Variable addition of sugars account for different IgG functional states. N-linked glycans at Asn-322 are noncore fucosylated and the vast majority are diantennary species with a bisecting GlcNAc. (Microbial infection) Deglycosylation on Asn-227 by S.pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway. O-linked glycans are non-, mono- and disialylated core 1-type O-glycans.

  • Subunit

    Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked

  • SwissProt ID

    P01860

  • Gene ID
    0 [NCBI]
  • Synonyms

    Growth hormone receptor; GH receptor; Somatotropin receptor

Human IgG3 Antibody (YA10021) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of IgG3 Fc Protein, Human (HEK293) using Human IgG3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Human IgG3 Antibody (YA10021) Product Image
    1/16
  • Experimental Validation Results for Human IgG3 Antibody (YA10021)
  • Human IgG3 Antibody (YA10021) Product Image
  • Experimental Validation Results for Human IgG3 Antibody (YA10021)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of IgG3 Fc Protein, Human (HEK293) using Human IgG3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Human IgG3 Antibody (YA10021)
  • Human IgG3 Antibody (YA10021) Product Image
    MedChemExpress Validation

    Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of IgG3 Fc Protein, Human (HEK293) using Human IgG3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Human IgG3 Antibody (YA10021) Product Image WB