PODXL Antibody (YA5023)

Cat. No.: HY-P85331: Data Sheet User Guide for Antibodies
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PODXL Antibody (YA5023) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PODXL.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

PODXL Antibody (YA5023) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to PODXL.

Host

Rabbit

Clonality

Monoclonal,Recombinant

분자량

Predicted band size: 59 kDa;

Observed band size: 165 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00592

Gene ID

5420 [NCBI]

Immunogen

A synthesized peptide derived from human Podocalyxin. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
IP IP: Immunoprecipitation	1:20-50
FC FC: Flow Cytometry	1:50-100

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells

Subcellular Localization：Apical cell membrane; Cell projection, lamellipodium; Cell projection, filopodium; Cell projection, ruffle; Cell projection, microvillus; Membrane raft; Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Glomerular epithelial cells (podocytes)

Isoforms & Post-Translational Modification：O00592 has 2 isomers: O00592-1: 58635 Da (predicted); O00592-2: 55386 Da (predicted).
N- and O-linked glycosylated. Sialoglycoprotein (By similarity)

Subunit：Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF1 (via the PDZ domains); the interaction is not detected in glomerular epithelium cells, take place early in the secretory pathway and is necessary for its apical membrane sorting. Found in a complex with EZR, PODXL and NHERF2. Associates with the actin cytoskeleton through complex formation with EZR and NHERF2. Interacts (via the C-terminal PDZ-binding motif DTHL) with NHERF2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells (By similarity). Interacts with EZR

Synonyms

PODXL; PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1

각종 서류

User Guide for Antibodies (1077 KB)