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TSPAN8 Antibody (YA3849)

(Synonyms: CO-029; TM4SF3)
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Based on 1 Customer Validation

TSPAN8 Antibody (YA3849) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN8.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • TSPAN8 Antibody (YA3849) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

TSPAN8 Antibody (YA3849) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TSPAN8.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Immunogen

Purified recombinant fragment of human TSPAN8 (AA: Extra(110-205)) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
    Western blot analysis of extracts from HT-29(lane 1(40μg) ) using TSPAN8 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling TSPAN8 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    TSPAN8 is a Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking. Promotes ADAM17-mediated TNF-alpha processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration. Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR. Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration. Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin[1][2][3][4][5][6].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Gastric, colon, rectal, and pancreatic carcinomas

  • Subunit

    Forms homooligomers (PubMed:34524408). Interacts with MEP1B (PubMed:27180357). Interacts with integrin alpha3/ITGA3 (PubMed:25761241). Interacts with RICTOR and MTOR (PubMed:25761241, PubMed:35904232). Interacts with ADAM17 (PubMed:36078095). Interacts with ECE1 (PubMed:37835445)

  • SwissProt ID

    P19075

  • Gene ID
    7103 [NCBI]
  • Synonyms

    CO-029; TM4SF3

References

[1]. Schmidt F, et al. Tetraspanin 8 is an interactor of the metalloprotease meprin β within tetraspanin-enriched microdomains. Biol Chem. 2016 May 14;:.

[2]. Müller M, et al. Tetraspanin 8 Subfamily Members Regulate Substrate-Specificity of a Disintegrin and Metalloprotease 17. Cells. 2022 Aug 29;11(17):.

[3]. Pan SJ, et al. Tetraspanin 8-rictor-integrin α3 complex is required for glioma cell migration. Int J Mol Sci. 2015 Mar 9;16(3):5363-74. [Content Brief]

[4]. Zhuang L, et al. TSPAN8 alleviates high glucose-induced apoptosis and autophagy via targeting mTORC2. Cell Biol Int. 2022 Oct;46(10):1693-1703. [Content Brief]

[5]. Min J, et al. Tetraspanin Tspan8 restrains interferon signaling to stabilize intestinal epithelium by directing endocytosis of interferon receptor. Cell Mol Life Sci. 2023 May 19;80(6):154.

[6]. Zhu Y, et al. The Tetraspanin Tspan8 Associates with Endothelin Converting Enzyme ECE1 and Regulates Its Activity. Cancers (Basel). 2023 Sep 27;15(19):. [Content Brief]

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TSPAN8 Antibody (YA3849) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HT-29(lane 1(40μg) ) using TSPAN8 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling TSPAN8 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TSPAN8 Antibody (YA3849) Product Image
    1/16
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • TSPAN8 Antibody (YA3849) Product Image
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HT-29(lane 1(40μg) ) using TSPAN8 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling TSPAN8 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • Experimental Validation Results for TSPAN8 Antibody (YA3849)
  • TSPAN8 Antibody (YA3849) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HT-29(lane 1(40μg) ) using TSPAN8 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH，1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TSPAN8 Antibody (YA3849) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling TSPAN8 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TSPAN8 Antibody (YA3849) Product Image WB
  • TSPAN8 Antibody (YA3849) Product Image FC