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KLF4 Antibody (YA716)

(Synonyms: Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4; Epithelial zinc finger protein EZF; Gut enriched Krueppel)
Customer Review

Based on 1 Customer Validation

KLF4 Antibody (YA716) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLF4.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KLF4 Antibody (YA716)
  • KLF4 Antibody (YA716) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

KLF4 Antibody (YA716) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLF4.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55/65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human KLF4.The exact sequence is proprietary to MCE.

Sensitivity

Transfected

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3102964

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KLF4 Antibody (YA716)
    Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Rat testis tissu (lane4(20μg) and Mouse testis tissue (lane5(20μg) using KLF4 Antibody (HY-P80735). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Isoforms & Post-Translational Modification

    O43474 has 5 isomers: O43474-3: 54671 Da (predicted); O43474-1: 51076 Da (predicted); O43474-4: 45639 Da (predicted); O43474-5: 12329 Da (predicted); O43474-6: 6489 Da (predicted).
    Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis;Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis

  • Subunit

    Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain) (PubMed:17308127). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a (PubMed:21746878). Interacts with ZNF296 (By similarity). Interacts with GLIS1 (PubMed:21654807). Interacts with BTRC; this interaction leads to KLF4 ubiquitination and subsequent degradation (By similarity). Interacts with IPO7; the interaction facilitates nuclear translocation of KLF4 in dental papilla cells (By similarity)

  • SwissProt ID

    O43474

  • Gene ID
    9314 [NCBI]
  • Synonyms

    Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4; Epithelial zinc finger protein EZF; Gut enriched Krueppel

  • Research Field

    Epigenetics and Nuclear Signaling

KLF4 Antibody (YA716) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Rat testis tissu (lane4(20μg) and Mouse testis tissue (lane5(20μg) using KLF4 Antibody (HY-P80735). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • KLF4 Antibody (YA716) Product Image
    1/16
  • Experimental Validation Results for KLF4 Antibody (YA716)
  • KLF4 Antibody (YA716) Product Image
  • Experimental Validation Results for KLF4 Antibody (YA716)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Rat testis tissu (lane4(20μg) and Mouse testis tissue (lane5(20μg) using KLF4 Antibody (HY-P80735). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KLF4 Antibody (YA716)
  • KLF4 Antibody (YA716) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Rat testis tissu (lane4(20μg) and Mouse testis tissue (lane5(20μg) using KLF4 Antibody (HY-P80735). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • KLF4 Antibody (YA716) Product Image WB