KLF4 Antibody (YA716)

Cat. No.: HY-P80735: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

KLF4 Antibody (YA716) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLF4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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KLF4 Antibody (YA716) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

KLF4 Antibody (YA716) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to KLF4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55/65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43474

Gene ID

9314 [NCBI]

Immunogen

Synthetic peptide corresponding to Human KLF4.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Transfected	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HCT116 (lane2(20μg), K562 (lane3(20μg), Rat testis tissu (lane4(20μg) and Mouse testis tissue (lane5(20μg) using KLF4 Antibody (HY-P80735). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription

Subcellular Localization：Nucleus; Cytoplasm

Isoforms & Post-Translational Modification：O43474 has 5 isomers: O43474-3: 54671 Da (predicted); O43474-1: 51076 Da (predicted); O43474-4: 45639 Da (predicted); O43474-5: 12329 Da (predicted); O43474-6: 6489 Da (predicted).
Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis;Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis

Subunit：Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain) (PubMed:17308127). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a (PubMed:21746878). Interacts with ZNF296 (By similarity). Interacts with GLIS1 (PubMed:21654807). Interacts with BTRC; this interaction leads to KLF4 ubiquitination and subsequent degradation (By similarity). Interacts with IPO7; the interaction facilitates nuclear translocation of KLF4 in dental papilla cells (By similarity)

RRID

AB_3102964

Database

Entrez Gene: 9314 Human ; 16600 Mouse ; 114505 Rat

SwissProt: O43474 Human ; Q60793 Mouse ;

OMIM: 602253 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4; Epithelial zinc finger protein EZF; Gut enriched Krueppel

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

KLF4 Antibody (YA716) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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KLF4 Antibody (YA716)

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KLF4 Antibody (YA716) Related Classifications

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