CD163 Antibody (YA4357)(PBS only)

Cat. No.: HY-P84660A: Data Sheet User Guide for Antibodies
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CD163 Antibody (YA4357) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD163.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD163 Antibody (YA4357) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD163.

Host

Mouse

Molecular Weight

Predicted band size: 125 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit, Monkey

SwissProt ID

Q86VB7

Gene ID

9332 [NCBI]

Immunogen

Purified recombinant fragment of human CD163 (AA: extra 42-259) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells; After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions

Subcellular Localization：Secreted; Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:This protein is expressed in monocytes and mature macrophages, such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident macrophages in the bone marrow, and meningeal macrophages in the central nervous system. It is also expressed in the blood. Isotype 1 is present in the lowest concentration in the blood. Isotype 2 is present in the lowest concentrations in the liver and spleen. Isotype 3 is the predominant isotype detected in the blood.

Induction:Induced by anti-inflammatory mediators such as glucocorticoids, interleukin-6/IL6 and interleukin-10/IL10; suppressed by pro-inflammatory mediators like bacterial lipopolysaccharides (LPS) , IFNG/IFN-gamma and TNF

Isoforms & Post-Translational Modification：Q86VB7 has 4 isomers: Q86VB7-1: 125451 Da (predicted); Q86VB7-2: 125982 Da (predicted); Q86VB7-3: 121609 Da (predicted); Q86VB7-4: 124958 Da (predicted).
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs;Phosphorylated

Subunit：Interacts with CSNK2B

Synonyms

M130; MM130; SCARI1

Documentation

User Guide for Antibodies (1077 KB)