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ST-tag II Antibody (YA885)

(Synonyms: ST-tag II; ST-tag )
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Based on 1 Customer Validation

ST-tag II Antibody (YA885) is an unconjugated, rabbit-derived, anti-ST-tag II (YA885) IgG monoclonal antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ST-tag II Antibody (YA885)
  • ST-tag II Antibody (YA885) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:10000-30000 1:2500 1-2μg/sample

Product Details

Description

ST-tag II Antibody (YA885) is an unconjugated, rabbit-derived, anti-ST-tag II (YA885) IgG monoclonal antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Immunogen

Synthetic peptide corresponding to ST-tag II.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103038

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ST-tag II Antibody (YA885)
    Western blot analysis of extracts from HY-P701340(lane 2(20ng) and HY-P701340 (lane 3(40ng) using ST-tag II (HY-P81039) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ST-tag II is a Strep-Tag II is a short peptide tag composed of 8 amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), exhibiting high specificity and affinity for streptavidin. It is commonly used for affinity purification, detection, and protein-protein interaction studies of recombinant proteins.

  • Synonyms

    ST-tag II; ST-tag

  • Research Field

    Tags & Cell Markers

ST-tag II Antibody (YA885) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HY-P701340(lane 2(20ng) and HY-P701340 (lane 3(40ng) using ST-tag II (HY-P81039) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ST-tag II Antibody (YA885) Product Image
    1/16
  • Experimental Validation Results for ST-tag II Antibody (YA885)
  • ST-tag II Antibody (YA885) Product Image
  • Experimental Validation Results for ST-tag II Antibody (YA885)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HY-P701340(lane 2(20ng) and HY-P701340 (lane 3(40ng) using ST-tag II (HY-P81039) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ST-tag II Antibody (YA885)
  • ST-tag II Antibody (YA885) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HY-P701340(lane 2(20ng) and HY-P701340 (lane 3(40ng) using ST-tag II (HY-P81039) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ST-tag II Antibody (YA885) Product Image WB