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ATM Antibody (YA5801)

(Synonyms: ATM; Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated)
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Based on 1 publication(s) in Google Scholar

ATM Antibody (YA5801) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATM.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATM Antibody (YA5801)
  • Experimental Validation Results for ATM Antibody (YA5801)
  • ATM Antibody (YA5801) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

ATM Antibody (YA5801) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATM.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 351 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 351 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719080

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATM Antibody (YA5801)
    Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATM Antibody (YA5801)
    Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ATM is a serine/threonine protein kinase and a crucial nexus for the cellular response to DNA double-stranded breaks[1]. Mechanistically, ATM signals to cell-cycle and DNA-repair components by phosphorylating downstream targets including p53, CHK2, NBS1, and BRCA1[2]. Transcription- and topoisomerase I-induced DNA double-strand breaks also activate ATM in post-mitotic neurons and lymphocytes, linking ATM signaling to transcription-associated genome stress[3]. In disease context, ATM mutations cause ataxia-telangiectasia, which includes neurodegeneration, immune dysfunction, radiosensitivity, and cancer predisposition[1]. Compared with related PIKK isoforms, ATM, ATR, and DNA-PK jointly control the DNA damage response, but ATR and DNA-PK can compensate for ATM absence in ataxia-telangiectasia[4]. DNA-PK differs functionally because DNA-PKcs and Ku are essential for double-strand break repair through non-homologous end joining[5]. For experimental applications, loss or inhibition of ATM activity increases sensitivity to ionizing radiation and chemotherapeutic agents that elicit DNA double-strand breaks[2]. KU55933 sensitized cells to the topoisomerase IIα inhibitor NK314, supporting ATM inhibition as a tool for studying DNA repair-dependent drug response[6].

  • Subcellular Localization

    Nucleus; Cytoplasmic vesicle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Peroxisome matrix

  • Expression


    Tissue_specificity:It is found in the pancreas, kidneys, skeletal muscle, liver, lungs, placenta, brain, heart, spleen, thymus, testes, ovaries, small intestine, colon, and white blood cells.

    Induction:By ionizing radiation

  • Subunit

    Homodimer (PubMed:12556884, PubMed:15790808, PubMed:28508083). Dimers or tetramers in inactive state (PubMed:12556884, PubMed:15790808, PubMed:28508083).

  • SwissProt ID

    Q13315

  • Gene ID
    472 [NCBI]
  • Synonyms

    ATM; Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated

References

[1]. McKinnon PJ. ATM and ataxia telangiectasia. EMBO Rep. 2004 Aug;5(8):772-6. doi: 10.1038/sj.embor.7400210. PMID: 15289825; PMCID: PMC1299121. et al. ATM and ataxia telangiectasia. EMBO Rep. 2004 Aug;5(8):772-6. [Content Brief]

[2]. Harnor S, et al. Modulation of the DNA-Damage Response by Inhibitors of the Phosphatidylinositol 3-Kinase Related Kinase (PIKK) Family[M]//Cancer II. Cham: Springer International Publishing, 2017: 189-189.

[3]. Sordet O, et al. Ataxia telangiectasia mutated activation by transcription- and topoisomerase I-induced DNA double-strand breaks. EMBO Rep. 2009 Aug;10(8):887-93. [Content Brief]

[4]. Schlam-Babayov S, et al. It takes three to the DNA damage response tango. Mol Cell Oncol. 2021 Feb 8;8(2):1881395. [Content Brief]

[5]. Matsumoto Y, et al. DNA-Dependent Protein Kinase Catalytic Subunit: The Sensor for DNA Double-Strand Breaks Structurally and Functionally Related to Ataxia Telangiectasia Mutated. Genes (Basel). 2021 Jul 27;12(8):1143. [Content Brief]

[6]. Guo L, et al. DNA-dependent protein kinase and ataxia telangiectasia mutated (ATM) promote cell survival in response to NK314, a topoisomerase IIα inhibitor. Mol Pharmacol. 2011 Aug;80(2):321-7. [Content Brief]

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ATM Antibody (YA5801) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATM Antibody (YA5801) Product Image
    1/16
  • Experimental Validation Results for ATM Antibody (YA5801)
  • Experimental Validation Results for ATM Antibody (YA5801)
  • ATM Antibody (YA5801) Product Image
  • Experimental Validation Results for ATM Antibody (YA5801)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATM Antibody (YA5801)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATM Antibody (YA5801)
  • Experimental Validation Results for ATM Antibody (YA5801)
  • ATM Antibody (YA5801) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATM Antibody (YA5801) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ATM with ATM Antibody (HY-P86109) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATM Antibody (HY-P86109) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATM Antibody (YA5801) Product Image ICC
  • ATM Antibody (YA5801) Product Image ICC