PRDM16 Antibody (YA7205)

Cat. No.: HY-P87521: Ficha de datos User Guide for Antibodies
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PRDM16 Antibody (YA7205) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to PRDM16.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn
Referencias

Descripciòn

PRDM16 Antibody (YA7205) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to PRDM16.

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 150 kDa;

Species Reactivity

Human

SwissProt ID

Q9HAZ2

Immunogen

Recombinant human PRDM16 between 779-996 amino acids.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Pureza	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1,k

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (By similarity). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (By similarity). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (By similarity). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (By similarity). Functions as a repressor of TGF-beta signaling (PubMed:19049980)|Binds DNA and functions as a transcriptional regulator (PubMed:12816872). Functions as a repressor of TGF-beta signaling (PubMed:14656887). May regulate granulocyte differentiation (PubMed:12816872)

Subcellular Localization：Nucleus,Cytoplasm

Expression：
Tissue_Specificity: Expressed in uterus and kidney. Expressed in both cardiomyocytes and interstitial cells

Isoforms & Post-Translational Modification：Q9HAZ2 has four isomers: Q9HAZ2-1: 140251 Da (predicted); Q9HAZ2-2: 138177 Da (predicted); Q9HAZ2-3: 140263 Da (predicted); Q9HAZ2-4: 120338 Da (predicted).

Subunit：Interacts with CEBPA, CEBPB and CEBPD; the interaction is direct

Documentación

User Guide for Antibodies (1077 KB)

Referencias

[1]. /biology-dictionary/a549-cell.html [Content Brief]