Islet 1 Antibody (YA4925)
(Synonyms: ISL1; Insulin gene enhancer protein ISL-1; Islet-1)
Based on 1 Customer Validation
Islet 1 Antibody (YA4925) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Islet 1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB; ICC/IF; IP; IHC-P; IHC-F; IF-Tissue
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
|
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
IHC-F
IHC-F: Immunohistochemistry-Frozen
|
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
|
|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 | 1:20-50 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
Islet 1 Antibody (YA4925) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Islet 1.
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 39 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 39 kDa
A synthesized peptide derived from human Islet 1. The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Islet 1 is a DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart
-
Subcellular Localization
Nucleus
-
Expression
Tissue_specificity:This gene is expressed in some neurons of the adrenal medulla and dorsal root ganglion, the nuclear layer and ganglion cell layer of the retina, the pineal gland, and certain areas of the brain. -
Subunit
At neuronal promoters, displaces LDB1 from LHX3 LIM domain to form a ternary complex in which ISL1 contacts both LHX3 and LDB1; allosteric structural changes in the DNA binding domain of LHX3, induced by the ISL1:LHX3 interaction, may explain differences in sequence specificity of the different complexes. Interacts with LHX3. Interacts (via C-terminus) with POU4F2 (via C-terminus) isoform 1. Interacts with POU3F2. Interacts with POU4F3. Interacts (via N-terminal domain) with MLIP; the interaction represses ISL1 transactivator activity. Interacts with GCN5/KAT2A. Interactions of ISL1 with MLIP1 or KAT2A may be mutually exclusive (By similarity)
-
SwissProt ID
-
Synonyms
ISL1; Insulin gene enhancer protein ISL-1; Islet-1
Documentation
Powered by Bioz
