Glucagon Antibody (YA4875)

Cat. No.: HY-P85183: Data Sheet User Guide for Antibodies
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Glucagon Antibody (YA4875) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Glucagon.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Glucagon Antibody (YA4875) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Glucagon.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 21 kDa;

Species Reactivity

Human

SwissProt ID

P01275

Gene ID

2641 [NCBI]

Immunogen

Purified recombinant fragment of human GCG expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes; Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6 (PubMed:22037645). Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis (Probable); Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability; Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life

Subcellular Localization：Secreted; Secreted

Expression：
Tissue_specificity:Secreted by pancreatic A cells; secreted by pancreatic A cells (PubMed:22037645) . Secreted by enteroendocrine L cells distributed throughout the gastrointestinal tract (PubMed:22037645) . Also secreted by certain neurons in the brain; secreted by enteroendocrine cells distributed throughout the gastrointestinal tract. Also secreted by certain neurons in the brain; secreted by enteroendocrine cells distributed throughout the gastrointestinal tract; secreted by enteroendocrine cells distributed throughout the gastrointestinal tract.

Induction:Release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin; Production by islet alpha cell is increased by IL6; Induced in response to nutrient ingestion

Synonyms

GCG; Glucagon

Documentation

User Guide for Antibodies (1077 KB)