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ENO1 Antibody (YA773)(PBS only)

(Synonyms: NNE; PPH; MPB1; ENO1L1; HEL-S-17)

ENO1 Antibody (YA773) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ENO1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ENO1 Antibody (YA773)(PBS only)
  • ENO1 Antibody (YA773)(PBS only) Product Image
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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ENO1 Antibody (YA773) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ENO1.

  • Host Mouse
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Immunogen

Synthetic peptide corresponding to Human Enolase-1 aa1-434.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENO1 Antibody (YA773)(PBS only)
    Western blot analysis of extracts from C6(lane 2(20ug) ,Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ENO1 Antibody (HY-P80655) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ENO1 is a Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production; Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor[1][2][3][4][5].

  • Subcellular Localization

    Cytoplasm; Cell membrane; Cytoplasm, myofibril, sarcomere, M line; Nucleus

  • Expression


    Tissue_specificity:The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons

    Induction:Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level)

  • Isoforms & Post-Translational Modification

    P06733 has 2 isomers: P06733-1: 47169 Da (predicted); P06733-2: 36928 Da (predicted).
    ISGylated;Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity

  • Subunit

    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific (PubMed:18560153). ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (PubMed:9308760). Isoform MBP-1 interacts with TRAPPC2B (PubMed:11134351). Interacts with ENO4 and PGAM2 (By similarity). Interacts with CMTM6 (PubMed:28813417)

  • SwissProt ID

    P06733

  • Gene ID
    2023 [NCBI]
  • Synonyms

    NNE; PPH; MPB1; ENO1L1; HEL-S-17

  • Research Field

    Signal Transduction

References

[1]. Sugahara T, et al. Purification and characterization of immunoglobulin production stimulating factor-II beta derived from Namalwa cells. Cytotechnology. 1992;10(2):137-46. [Content Brief]

[2]. Huang H, et al. p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis. Mol Cell. 2018 May 17;70(4):663-678.e6. [Content Brief]

[3]. Feo S, et al. ENO1 gene product binds to the c-myc promoter and acts as a transcriptional repressor: relationship with Myc promoter-binding protein 1 (MBP-1). FEBS Lett. 2000 May 4;473(1):47-52. [Content Brief]

[4]. López-Alemany R, et al. Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase. Am J Hematol. 2003 Apr;72(4):234-42. [Content Brief]

[5]. Ray R, et al. Cloning and characterization of a human c-myc promoter-binding protein. Mol Cell Biol. 1991 Apr;11(4):2154-61. [Content Brief]

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ENO1 Antibody (YA773)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) ,Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ENO1 Antibody (HY-P80655) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ENO1 Antibody (YA773)(PBS only) Product Image
    1/16
  • Experimental Validation Results for ENO1 Antibody (YA773)(PBS only)
  • ENO1 Antibody (YA773)(PBS only) Product Image
  • Experimental Validation Results for ENO1 Antibody (YA773)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) ,Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ENO1 Antibody (HY-P80655) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENO1 Antibody (YA773)(PBS only)
  • ENO1 Antibody (YA773)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) ,Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ENO1 Antibody (HY-P80655) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ENO1 Antibody (YA773)(PBS only) Product Image WB