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Beta Tubulin Antibody (YA839)

(Synonyms: Human,Mouse,Monkey,Goat,Hamster,Rat,ChlamydomonasReinhardtii)
Customer Review

Based on 1 Customer Validation

Beta Tubulin Antibody (YA839) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Tubulin beta chain (TUBB). It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas Reinhardtii

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Beta Tubulin Antibody (YA839) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:5000-1:10000 1:100-1:200

Product Details

Description

Beta Tubulin Antibody (YA839) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Tubulin beta chain (TUBB). It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas Reinhardtii
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 50 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Tubulin beta.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102772

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Beta Tubulin is a Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:This gene is widely expressed in all tissues of the body, with the highest expression levels in the spleen, thymus, and immature brain tissue.

    Induction:Autoregulated by feedback control of mRNA degradation (PubMed:31727855) . In excess of soluble tubulin, nascent beta-tubulin chain binds TTC5/STRAP cofactor through the MREI motif which triggers cotranslation degradation of tubulin mRNA (PubMed:31727855)

  • Subunit

    Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CIMAP3 (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity). Nascent tubulin polypeptide interacts (via beta-tubulin MREI motif) with TTC5/STRAP; this interaction results in tubulin mRNA-targeted degradation (PubMed:31727855)

  • SwissProt ID

    P07437

  • Gene ID
    203068 [NCBI]
  • Synonyms

    Human,Mouse,Monkey,Goat,Hamster,Rat,ChlamydomonasReinhardtii

  • Research Field

    Cell Biology

Beta Tubulin Antibody (YA839) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beta Tubulin Antibody (YA839) Product Image
    1/16
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Beta Tubulin Antibody (YA839) Product Image
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Experimental Validation Results for Beta Tubulin Antibody (YA839)
  • Beta Tubulin Antibody (YA839) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Beta Tubulin Antibody (YA839) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beta Tubulin Antibody (YA839) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Beta Tubulin Antibody (HY-P80955) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beta Tubulin Antibody (YA839) Product Image WB
  • Beta Tubulin Antibody (YA839) Product Image ICC
  • Beta Tubulin Antibody (YA839) Product Image ICC