Beta Tubulin Antibody (YA839)

Art. -Nr.: HY-P80955: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Beta Tubulin Antibody (YA839) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Tubulin beta chain (TUBB). It can be used as a loading control antibody.

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Größe	Verfügbarkeit	Menge
20 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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Beta Tubulin Antibody (YA839) Spezialitätsempfehlungen:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Beschreibung

Beschreibung

Beta Tubulin Antibody (YA839) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Tubulin beta chain (TUBB). It can be used as a loading control antibody.

Host

Mouse

Clonality

Monoclonal

Molekulargewicht

Predicted band size: 50 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas Reinhardtii

SwissProt ID

P07437

Gene ID

203068 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Tubulin beta.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:200

Sensitivity	Endogenous	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MDA-MB-231 (lane 1) and Raji (lane 2) and Hela (lane 3) and Ramos (lane 4) using beta Tubulin antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (GAPDH, HY-P80954A, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin

Subcellular Localization：Cytoplasm, cytoskeleton

Expression：
Tissue_specificity:This gene is widely expressed in all tissues of the body, with the highest expression levels in the spleen, thymus, and immature brain tissue.

Induction:Autoregulated by feedback control of mRNA degradation (PubMed:31727855) . In excess of soluble tubulin, nascent beta-tubulin chain binds TTC5/STRAP cofactor through the MREI motif which triggers cotranslation degradation of tubulin mRNA (PubMed:31727855)

Subunit：Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with CIMAP3 (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity). Nascent tubulin polypeptide interacts (via beta-tubulin MREI motif) with TTC5/STRAP; this interaction results in tubulin mRNA-targeted degradation (PubMed:31727855)

RRID

AB_3102772

Database

Entrez Gene: 203068 Human ; 22154 Mouse ; 29214 Rat

SwissProt: P07437 Human ; P99024 Mouse ; P69897 Rat

OMIM: 615771 Human

Research Field

Cell Biology

Synonyms

Human,Mouse,Monkey,Goat,Hamster,Rat,ChlamydomonasReinhardtii

Dokumentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Beta Tubulin Antibody (YA839) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Beta Tubulin Antibody (YA839)

Beta Tubulin Antibody (YA839) Spezialitätsempfehlungen:

Product Detail

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Background

Beschreibung

Beta Tubulin Antibody (YA839) Related Classifications

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