Annexin-4/ANXA4 Antibody (YA2714)(PBS only)

Cat. No.: HY-P82969A: Data Sheet User Guide for Antibodies
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Annexin-4/ANXA4 Antibody (YA2714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin-4/ANXA4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Annexin-4/ANXA4 Antibody (YA2714) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Annexin-4/ANXA4.

Host

Rabbit

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P09525

Gene ID

307 [NCBI]

Immunogen

Recombinant protein of human Annexin-4/ANXA4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100
FC FC: Flow Cytometry	1:200-1:500

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HepG2 (lane 3, 15 μg), Neuro-2a (lane 4, 15 μg), C2C12 (lane 5, 15 μg), and PC-12 (lane 6, 15 μg) using STUB1 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis

Subcellular Localization：Zymogen granule membrane; Peripheral membrane protein

Isoforms & Post-Translational Modification：P09525 has 3 isomers: P09525-1: 35883 Da (predicted); P09525-2: 27063 Da (predicted); P09525-3: 36085 Da (predicted).

Research Field

Cardiovascular

Synonyms

AIV; AnnexinA4; AnnexinIV; ANX 4; ANX A4; ANX4; ANXA4; Chromobindin4; EndonexinI; LipocortinIV; P32.5; P33/41; PAPII; PIG28; PP4X; Protein II; Xanx-4; ZAP36

Documentation

User Guide for Antibodies (1077 KB)