Phospho-FGFR3 (Tyr642) Antibody (YA10037)
(Synonyms: CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3)Phospho-FGFR3 (Tyr642) Antibody (YA10037) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-FGFR3 (Tyr642).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP, FC
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 | 1:10-50 | 1:10-50 |
Product Details
Phospho-FGFR3 (Tyr642) Antibody (YA10037) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-FGFR3 (Tyr642).
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 88 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 88 kDa
Synthetic Phospho-peptide corresponding to residues surrounding Y642 of human FGFR3.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FGFR3 is a Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling
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Subcellular Localization
Cell membrane; Cytoplasmic vesicle; Endoplasmic reticulum
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Expression
Tissue_Specificity: Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells. -
Isoforms & Post-Translational Modification
Phospho-FGFR3 has 4 isoforms, P22607-1: amino acid length is 806, molecular weight is 87710 Da (predicted); P22607-2: amino acid length is 808, molecular weight is 88157 Da (predicted); P22607-3: amino acid length is 694, molecular weight is 75696 Da (predicted); P22607-4: amino acid length is 791, molecular weight is 85083 Da (predicted).
Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-724 is essential for stimulation of cell proliferation and activation of PIK3R1, STAT1 and MAP kinase signaling. Phosphorylation at Tyr-760 is required for interaction with PIK3R1 and PLCG1. -
Subunit
Monomer.
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SwissProt ID
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Synonyms
CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3
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