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Rad18 Antibody (YA3067)

(Synonyms: E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18; RAD18; S. cerevisiae; homolog; RING finger protein 73; RNF73)
Customer Review

Based on 1 Customer Validation

Rad18 Antibody (YA3067) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad18.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Rad18 Antibody (YA3067) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Rad18 Antibody (YA3067) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad18.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 56 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human RAD18 aa50-100/495.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rad18 Antibody (YA3067)
    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), U-87MG (lane 3, 15 μg), PC-3 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using Rad18 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Rad18 Antibody (YA3067)
    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), PC-3 (lane 5(20μg) using Rad18 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Rad18 is an E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity

  • Subcellular Localization

    Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Subunit

    Homodimer (PubMed:21549715). Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with SHPRH (PubMed:17108083, PubMed:17130289). Interacts with HLTF (PubMed:18316726, PubMed:18719106). Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (PubMed:22681887). Interacts (via C-terminus and phosphorylated form) with SLF1 (via BRCT domains); this interaction is required for efficient repair of UV-induced DNA damage (PubMed:15632077, PubMed:22036607, PubMed:25931565). Interacts with SLF2 (PubMed:25931565). Interacts with SMC5; this interaction is increased in a SLF1 or SLF2-dependent manner (PubMed:25931565). Interacts with DNA damage up-regulated protein DDUP (PubMed:35849344). Forms a complex with DDUP and H2AX following DDUP phosphorylation (PubMed:35849344)

  • SwissProt ID

    Q9NS91

  • Gene ID
    56852 [NCBI]
  • Synonyms

    E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18; RAD18; S. cerevisiae; homolog; RING finger protein 73; RNF73

  • Research Field

    Epigenetics and Nuclear Signaling

Rad18 Antibody (YA3067) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), U-87MG (lane 3, 15 μg), PC-3 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using Rad18 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), PC-3 (lane 5(20μg) using Rad18 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Rad18 Antibody (YA3067) Product Image
    1/16
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Rad18 Antibody (YA3067) Product Image
  • Experimental Validation Results for Rad18 Antibody (YA3067)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), U-87MG (lane 3, 15 μg), PC-3 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using Rad18 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Rad18 Antibody (YA3067)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), PC-3 (lane 5(20μg) using Rad18 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Experimental Validation Results for Rad18 Antibody (YA3067)
  • Rad18 Antibody (YA3067) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), U-87MG (lane 3, 15 μg), PC-3 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using Rad18 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Rad18 Antibody (YA3067) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), PC-3 (lane 5(20μg) using Rad18 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Rad18 Antibody (YA3067) Product Image WB
  • Rad18 Antibody (YA3067) Product Image WB