Rad18 Antibody (YA3067)

Cat. No.: HY-P83322: Data Sheet COA
SDS
User Guide for Antibodies
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Rad18 Antibody (YA3067) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad18.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Rad18 Antibody (YA3067) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Rad18 Antibody (YA3067) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad18.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 56 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NS91

Gene ID

56852 [NCBI]

Immunogen

A synthesized peptide derived from human RAD18 aa50-100/495.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from 293T (lane 1, 15 μg), Hela (lane 2, 15 μg), U-87MG (lane 3, 15 μg), PC-3 (lane 4, 15 μg), 3T3 (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using Rad18 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), MCF-7 (lane 3(20μg), Hela (lane 4(20μg), PC-3 (lane 5(20μg) using Rad18 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity

Subcellular Localization：Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Subunit：Homodimer (PubMed:21549715). Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with SHPRH (PubMed:17108083, PubMed:17130289). Interacts with HLTF (PubMed:18316726, PubMed:18719106). Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (PubMed:22681887). Interacts (via C-terminus and phosphorylated form) with SLF1 (via BRCT domains); this interaction is required for efficient repair of UV-induced DNA damage (PubMed:15632077, PubMed:22036607, PubMed:25931565). Interacts with SLF2 (PubMed:25931565). Interacts with SMC5; this interaction is increased in a SLF1 or SLF2-dependent manner (PubMed:25931565). Interacts with DNA damage up-regulated protein DDUP (PubMed:35849344). Forms a complex with DDUP and H2AX following DDUP phosphorylation (PubMed:35849344)

Database

Entrez Gene: 56852 Human ; 58186 Mouse ; 362412 Rat

SwissProt: Q9NS91 Human ; Q9QXK2 Mouse ;

OMIM: 605256 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18; RAD18; S. cerevisiae; homolog; RING finger protein 73; RNF73

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Rad18 Antibody (YA3067) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rad18 Antibody (YA3067)

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Background

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Rad18 Antibody (YA3067) Related Classifications

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