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  5. Muscarinic Acetylcholine receptor M3 Antibody (YA3525)

Muscarinic Acetylcholine receptor M3 Antibody (YA3525)

Cat. No.: HY-P81142
COA
SDS
User Guide for Antibodies Technical Support

Muscarinic Acetylcholine receptor M3 Antibody (YA3525) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Muscarinic Acetylcholine receptor M3.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈 가격 재고 수량
10 μL 해외재고보유
50 μL 해외재고보유
100 μL 해외재고보유
250 μL   견적 받기  
or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • 제품 설명

제품 설명

Muscarinic Acetylcholine receptor M3 Antibody (YA3525) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Muscarinic Acetylcholine receptor M3.
Host

Rabbit
Clonality

Monoclonal,Recombinant
분자량
Predicted band size: 66 kDa;
Observed band size: 66 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3 aa22-88.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), PC-3 (lane 2, 15 μg), and HT-29 (lane 3, 15 μg) using CHRM3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta Actin, HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover
Subcellular Localization：Cell membrane; Multi-pass membrane protein; Postsynaptic cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Endoplasmic reticulum membrane; Multi-pass membrane protein
Expression：
Tissue_specificity:Expressed in cerebral cortex, submandibular gland, hypothalamus, pancreas, liver, and ileum
Subunit：Homodimer; the dimers can form tetramers (By similarity). Interacts with NALCN (PubMed:19575010). Interacts with TMEM147 (By similarity)
RRID
Database

Entrez Gene: 12671 Human

SwissProt: Q9ERZ3 Human

Synonyms
Muscarinic Acetylcholine receptor M3; cholinergic receptor, muscarinic 3; CHRM 3; CHRM3; HM 3; HM3; HM3; EGBRS; M3 muscarinic receptor; muscarinic 3; m3 muscarinic acetylcholine receptor; ACM3_HUMAN; muscarinic receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor; Acetylcholine receptor(M3).
각종 서류
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Muscarinic Acetylcholine receptor M3 Antibody (YA3525) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
Muscarinic Acetylcholine receptor M3 Antibody (YA3525)
Cat. No.:
HY-P81142
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SAFETY DATA SHEET (SDS)

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