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CD44 Antibody (YA534)

(Synonyms: LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; Hyaluronate receptor antibody; IN antibody; MC56 antibody; MDU2 antibody; MDU3 antibody)
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Based on 1 publication(s) in Google Scholar

CD44 Antibody (YA534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD44.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:100

Product Details

Description

CD44 Antibody (YA534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD44.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 75-82 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human CD44.AA range:150-190.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102288

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD44 Antibody (YA534)
    Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640)

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, microvillus; Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in fibroblasts and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . Protein levels were also detected in the placenta (PubMed:32337544) . Isomer 10 (epithelial isomer) is expressed in epithelial cells and is highly expressed in cancer cells. Expression is suppressed in neuroblastoma cells.

  • Subunit

    Interacts with PKN2 (PubMed:15123640). Interacts with TIAM1 and TIAM2 (By similarity). Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment (PubMed:14992719, PubMed:17085435, PubMed:25195884). Interacts with UNC119 (PubMed:19381274). Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration (PubMed:20962267). Interacts with RDX, EZR and MSN (By similarity). Interacts with EGFR (PubMed:18757307, PubMed:23589287). Interacts with CD74; this complex is essential for the MIF-induced signaling cascade that results in B cell survival (By similarity)

  • SwissProt ID

    P16070

  • Gene ID
    960 [NCBI]
  • Synonyms

    LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; Hyaluronate receptor antibody; IN antibody; MC56 antibody; MDU2 antibody; MDU3 antibody

  • Research Field

    Immunology

CD44 Antibody (YA534) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD44 Antibody (YA534) Product Image
    1/16
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD44 Antibody (YA534) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD44 Antibody (YA534) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD44 Antibody (YA534) Product Image WB
  • CD44 Antibody (YA534) Product Image WB
  • CD44 Antibody (YA534) Product Image FC