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CD44 Antibody (YA534)

(Synonyms: LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; Hyaluronate receptor antibody; IN antibody; MC56 antibody; MDU2 antibody; MDU3 antibody)
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Based on 1 publication(s) in Google Scholar

CD44 Antibody (YA534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD44.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:100

Product Details

Description

CD44 Antibody (YA534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD44.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 75-82 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 82 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human CD44.AA range:150-190.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102288

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD44 Antibody (YA534)
    Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CD44 is a hyaluronic acid receptor and cell adhesion molecule that plays a broad role in cell migration, immune cell activation, stem cell maintenance, and tumorigenesis. CD44 serves as both a key marker for memory T cells and a classic surface marker in research on mesenchymal stem cells and cancer stem cells.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, microvillus; Secreted

  • Expression


    Tissue_specificity:Protein levels were detected in fibroblasts and urine (PubMed:25326458, PubMed:36213313, PubMed:37453717) . Protein levels were also detected in the placenta (PubMed:32337544) . Isomer 10 (epithelial isomer) is expressed in epithelial cells and is highly expressed in cancer cells. Expression is suppressed in neuroblastoma cells.

  • Isoforms & Post-Translational Modification

    CD44 has 19 isomers: P16070-1: 81538 Da (predicted); P16070-2: 3327 Da (predicted); P16070-3: 77983 Da (predicted); P16070-4: 76612 Da (predicted); P16070-5: 80790 Da (predicted); P16070-6: 76705 Da (predicted); P16070-7: 78446 Da (predicted); P16070-8: 74388 Da (predicted); P16070-9: 74196 Da (predicted); P16070-10: 53411 Da (predicted); P16070-11: 46565 Da (predicted); P16070-12: 39416 Da (predicted); P16070-13: 46261 Da (predicted); P16070-14: 43169 Da (predicted); P16070-15: 32075 Da (predicted); P16070-16: 73150 Da (predicted); P16070-17: 75957 Da (predicted); P16070-18: 37278 Da (predicted); P16070-19: 15635 Da (predicted).
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors;N-glycosylated;O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638;Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672

  • Subunit

    Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with UNC119. Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration. Interacts with EGFR.

  • SwissProt ID

    P16070

  • Gene ID
    960 [NCBI]
  • Synonyms

    LHR antibody; BA-1 antibody; CD 44 antibody; CD44 antibody; CD44 antigen antibody; CD44 molecule (Indian blood group) antibody; CD44 molecule antibody; CD44_HUMAN antibody; CDw44 antibody; Cell surface glycoprotein CD44 antibody; chondroitin sulfate proteoglycan 8 antibody; CSPG8 antibody; ECMR-III antibody; Epican antibody; Extracellular matrix receptor III antibody; GP90 lymphocyte homing/adhesion receptor antibody; HCELL antibody; hematopoietic cell E- and L-selectin ligand antibody; Heparan sulfate proteoglycan antibody; Hermes antigen antibody; homing function and Indian blood group system antibody; HSA antibody; HUTCH-I antibody; HUTCH1 antibody; Hyaluronate receptor antibody; IN antibody; MC56 antibody; MDU2 antibody; MDU3 antibody

  • Research Field

    Immunology

CD44 Antibody (YA534) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD44 Antibody (YA534) Product Image
    1/16
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD44 Antibody (YA534)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • Experimental Validation Results for CD44 Antibody (YA534)
  • CD44 Antibody (YA534) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD44 Antibody (YA534) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MDA-MB-231(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using CD44 Antibody (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD44 Antibody (YA534) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling CD44 Antibody(HY-P80062, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CD44 Antibody (YA534) Product Image WB
  • CD44 Antibody (YA534) Product Image WB
  • CD44 Antibody (YA534) Product Image FC