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  5. Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only)

Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only)

製品番号: HY-P81749A
COA User Guide for Antibodies Technical Support

Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p27 Kip 1 (Ser10).

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容量 価格（税別） 在庫状況 数量
10 μL $209 在庫あり
50 μL $540 在庫あり
100 μL $870 在庫あり
250 μL   お問い合わせ  
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* アイテムを追加する前、数量をご選択ください

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • 説明

製品説明

Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p27 Kip 1 (Ser10).
Host

Rabbit
Clonality

Monoclonal,Recombinant
分子量
Predicted band size: 22 kDa;
Observed band size: 27 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous 純度 Affinity Purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
輸送条件

Shipping with blue ice.
Background
Function：Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry
Subcellular Localization：Nucleus; Cytoplasm; Endosome
Expression：
Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .

Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase
Subunit：Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.
RRID
Research Field

Cell Biology
Synonyms
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
ドキュメンテーション

COA (205 KB)

User Guide for Antibodies (1077 KB)

Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
Phospho-p27 Kip 1 (Ser10) Antibody (YA1494) (PBS only)
製品番号:
HY-P81749A
数量:
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