RAB35 Antibody
(Synonyms: RAB1C, RAY, RAB35, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C)RAB35 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB35.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
RAB35 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB35.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 23 kDa
Synthetic peptide corresponding to the center region of human RAB35.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RAB35 is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB35 is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes (By similarity)[1][2][3].
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Subcellular Localization
Cell membrane; Membrane, clathrin-coated pit; Cytoplasmic vesicle, clathrin-coated vesicle; Endosome; Melanosome
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Isoforms & Post-Translational Modification
RAB35 has 2 isoforms, Q15286-1: amino acid length is 201, molecular weight is 23025 Da (predicted); Q15286-2: amino acid length is 152, molecular weight is 17058 Da (predicted).LRRK2 介导的第 72 位苏氨酸(Thr-72)磷酸化会阻止其与调控蛋白结合，包括 CHM、CHML 以及 GDP 解离抑制剂 GDI1 和 GDI2RAB35 存在 2 个异构体，Q15286-1：氨基酸个数为 201 个，分子量为 23025 Da (预测)；Q15286-2：氨基酸个数为 152 个，分子量为 17058 Da (预测)。Phosphorylation at Thr-72 by LRRK2 prevents the association of regulatory proteins including CHM, CHML and GDP dissociation inhibitors GDI1 and GDI2
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Subunit
Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner.
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SwissProt ID
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Synonyms
RAB1C, RAY, RAB35, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C
Documentation
[1]. Lin L, et al. Rab35/ACAP2 and Rab35/RUSC2 Complex Structures Reveal Molecular Basis for Effector Recognition by Rab35 GTPase. Structure. 2019 May 7;27(5):729-740.e3. [Content Brief]
[2]. Rahajeng J, et al. MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a. Traffic. 2012 Jan;13(1):82-93. [Content Brief]
[3]. Kouranti I, et al. Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis. Curr Biol. 2006 Sep 5;16(17):1719-25. [Content Brief]
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