Copy Link Print Information

FACL4 Antibody (YA6157)

(Synonyms: Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACS4 antibody; ACSL 4 antibody; Acsl4 antibody)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

FACL4 Antibody (YA6157) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FACL4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • FACL4 Antibody (YA6157) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) FACL4 Antibody (YA6157)

More

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:2000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

FACL4 Antibody (YA6157) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FACL4.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719166

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Western blot analysis of extracts from C6 (lane2(20μg), A549 (lane3(20μg), RAW264.7 (lane4(20μg) and 293T (lane5(20μg) using FACL4 Antibody (HY-P86465). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunocytochemistry analysis of Hela cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    Immunocytochemistry analysis of HepG2 cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    FACL4 catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion[1][2][3].

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass type III membrane protein; Peroxisome membrane; Single-pass type III membrane protein; Microsome membrane; Single-pass type III membrane protein; Endoplasmic reticulum membrane; Single-pass type III membrane protein; Cell membrane

  • Isoforms & Post-Translational Modification

    O60488 has 2 isomers: O60488-1: 79188 Da (predicted); O60488-2: 74436 Da (predicted).

  • SwissProt ID

    O60488

  • Gene ID
    2182 [NCBI]
  • Synonyms

    Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACS4 antibody; ACSL 4 antibody; Acsl4 antibody

FACL4 Antibody (YA6157) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2(20μg), A549 (lane3(20μg), RAW264.7 (lane4(20μg) and 293T (lane5(20μg) using FACL4 Antibody (HY-P86465). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FACL4 Antibody (YA6157) Product Image
    1/16
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • FACL4 Antibody (YA6157) Product Image
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2(20μg), A549 (lane3(20μg), RAW264.7 (lane4(20μg) and 293T (lane5(20μg) using FACL4 Antibody (HY-P86465). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FACL4 Antibody (YA6157)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • Experimental Validation Results for FACL4 Antibody (YA6157)
  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane2(20μg), A549 (lane3(20μg), RAW264.7 (lane4(20μg) and 293T (lane5(20μg) using FACL4 Antibody (HY-P86465). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using FACL4 Antibody (YA6157). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86465, 1/200) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FACL4 Antibody (YA6157) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling FACL4 with FACL4 Antibody (HY-P86465) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with FACL4 Antibody (HY-P86465) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • FACL4 Antibody (YA6157) Product Image WB
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image IHC-P
  • FACL4 Antibody (YA6157) Product Image ICC
  • FACL4 Antibody (YA6157) Product Image ICC